Evaluation of Stem Cell Components in Retrocorneal Membranes

다국어 초록 (Multilingual Abstract)

The purpose of this study was to elucidate the origin and cellular composition ofretrocorneal membranes (RCMs) associated with chemical burns usingimmunohistochemical staining for primitive cell markers. Six cases of RCMs were collectedduring penetrating keratoplasty. We examined RCMs with hematoxylin and eosin (H&E),periodic acid-Schiff (PAS) staining and immunohistochemical analysis using monoclonalantibodies against hematopoietic stem cells (CD34, CD133, c-kit), mesenchymal stem cells(beta-1-integrin, TGF-β, vimentin, hSTRO-1), fibroblasts (FGF-β, α-smooth muscle actin),and corneal endothelial cells (type IV collagen, CD133, VEGF, VEGFR1). Histologic analysisof RCMs revealed an organized assembly of spindle-shaped cells, pigment-laden cells, andthin collagenous matrix structures. RCMs were positive for markers of mesenchymal stemcells including beta-1-integrin, TGF-β, vimentin, and hSTRO-1. Fibroblast markers werealso positive, including FGF-β and α-smooth muscle actin (SMA). In contrast,immunohistochemical staining was negative for hematopoietic stem cell markers includingCD34, CD133 and c-kit as well as corneal endothelial cell markers such as type IV collagen,CD133 except VEGF and VEGFR1. Pigment-laden cells did not stain with any antibodies.
The results of this study suggest that RCMs consist of a thin collagen matrix and fibroblastlikecells and may be a possible neogenetic structure produced from a lineage of bonemarrow-derived mesenchymal stem cells.

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