Polyhydroxylated Alkaloids 생산균주의 분리 및 동정 = Isolation and Identification of Microorganism Producing Ployhydroxylated Alkaloids

다국어 초록 (Multilingual Abstract)

Over one hundred polyhydroxylated alkaloids have been isolated from various kinds of plants and microorganisms. The alkaloids are known as a potential inhibiting ability against glycosidase, which is involved in a wide range of important biological processes such as in the intestinal digestion, post-translational processing of glycoproteins and lysosomal catabolism of glycoconjugates. 1-Deoxynojirimycin(DNJ), one of the piperidine alkaloids, has been focused an its strong α-glucosidase-inhibiting activity as well as its potent suppressive reaction to the viral maturation. In recent, DNJ was particularly applied to the treatment of diabetes due to its reducing power of diet-induced hyperglycemia by inhibiting intestinal α-glucosidase. Thus, the available resources were investigated in order to get large amounts of DNJ. The bacterial strain was isolated MORI-91 from the cereal. Strain MORI-91 mainly produced DNJ as well as other polyhydroxylated alkaloids with high potency of α-glucosidase-inhibiting activity. A bacterium producing DNJ was identified as Bacillus subtilis by means of a quinone analysis, fatty acids composition analysis in a whole cell content, sugar utilization tests, a partial 16S rRNA sequencing data, and a scanning electron micrograph of cellular morphology.