Cassava 澱粉을 利用한 Rhizopus 및 Aspergillus niger에 關한 硏究 = (A) Study on the Utilization of Cassava Starch by a Strain of Rhizopus and Aspergillus niger

다국어 초록 (Multilingual Abstract)

Several species of the fungi were isolated from cassava starch which was produced and was formed into pellet shape.
The pellet was stored for a while in southern part of Thailand.
The species of Rhizopus Aspergillus niger, and Aspergillus fumigatus were identified through classification methods.
The experimental results are as follows;
1. Dry weight increases were checked during the static liquid culture with modified Czapek Dox medium to which cassava starch were partly replaced to sugar, Aspergillus niger and Aspergillus fumigatue were well grown than Rizopue species when 6% cassava starch was replaced to sugar and grown for 72 hours.
2. Amounts of mycelial protein were checked, the highest amount was shown in 6% cassava starch contained medium by Aspergillus niger.
3. When nitrogen sources were varied such as ammonium sulfate or Urea against sodium nitrate, there was no significant difference in mycelial production.
4. Alpha amylase activity of each fungus isolated here was checked, Asperpillus niger has shown the highest peak at 72 hours.

목차 (Table of Contents)

• 目次
• Ⅰ. 緖論 = 1
• Ⅱ. 實驗材料 및 方法 = 4
• 1. 實驗 材料 = 4
• 2. 試藥 및 機器 = 4
• 3. 實驗·方法 = 5
• 1) 곰팡이의 分離 = 5
• 2) 곰팡이의 同定 = 6
• 3) 分離된 곰팡이의 培養 = 6
• a. 接種用菌의 培養 = 6
• b. 胞子發芽用 盡湯培養 = 6
• 4) 곰팡이 培養液中의 澱粉液化 酵素液 = 7
• 5) 分離된 곰팡이의 Cassava 澱粉利用實驗 = 7
• a. Cassava 澱粉이 含有된 培地의 調製 = 7
• b. Cassava 澱粉의 液化處理 및 곰팡이의 培養 = 10
• c. 菌體量測定 및 一般成分分析 = 10
• ① 菌體量測定 = 10
• ② 水分測定 = 10
• ③ 灰分定量 = 12
• ④ 粗脂肪 定量 = 13
• ⑤ 總灰水化物定量 = 14
• d. 菌體蛋白質量의 測定 = 14
• ① Lowry 法 = 14
• ⅰ) 標準檢量曲線 作成 = 14
• ⅱ) 菌體蛋白質定量 = 14
• e. 菌體의 澱粉液化酵素 α-Amylase 活性測定 = 16
• Ⅲ. 實驗結果 = 19
• 1. 곰팡이의 分離同定 = 19
• 2. 菌體量의 測定 및 一般成分分析 = 19
• 3. 菌體蛋白質의 定量 = 19
• 4. 菌體의 澱粉液化 酵素 (α-Amylase) 活性測定 = 32
• 5. 走査電子顯微鏡 사진촬영 = 35
• Ⅳ. 考察 = 39
• Ⅴ. 結論 = 41
• 參考文獻 = 42
• ABSTRACT = 45