SDS-Polyacrylamide gel 電氣泳動에 의한 누에의 發育段階別 血蛋白質의 變化 = Changes of Haemolymph proteins during Metamorphosis of silkworm, Bombyx mori, by SDS-polyacrylamide gel electrophoresis

다국어 초록 (Multilingual Abstract)

This experiments were carried out to study changes of haemolymph proteins during development of silkworm, Bombyx mori by SDS-polyacrylamide gel electrophoresis.
The results obtained were follows;
1. Bands of haemolymph proteins seperated during the metamorphosis of silkworm showed 20 bands and those molecular weights ranged from 12,800 to 106,000 daltons.
2. Major bands (7 and 15) were changed quantitatively, band 7 showed predominant levels from the middle 5th instar to the middle pupa stage, band 15 was leached the maximum levels at spining and then significantly decreased on prepupa stage, those molecular weights were determined to be 27,000 and 79,000 daltons respectively.
3. Female specific proteins of band 17 and 18 (mol. wt. 102,000 and 102,500 daltons) showed from the 1st day after pupation.
4. Concentration of low molecular weight proteins (mol. wt. 16,300∼18,500 daltons) at adult stage were increased.
5. In conclusion, it was suggested that the major haemolymph proteins in silkworm should be utilized to form tissues of pupa and adult, female specific proteins involved in the egg formation.

목차 (Table of Contents)

• Ⅰ. 緖論
• Ⅱ. 實驗材料 및 方法
• 1. 實驗材料
• 2. 實驗方法
• Ⅲ. 結果
• Ⅳ. 考察
• Ⅴ. 摘要