염색체 분석에 의한 생쥐 분할란의 성감별 = Sexing Mouse Demi - embryos by Chromosomal Analysis

다국어 초록 (Multilingual Abstract)

These experiments were carried out to obtain basic information necessary to produce the offsprings which were sexed by chromosomal analysis prior to transfer in the mouse. Morula stage embryos were obtained at 3 clays post-coitum. Embryos were mechanically bisected by a glass microneedle and then the pairs of demi-embryos were cultured in BMOC-3 medium under the humidified atmosphere of 5% CO₂ at 37℃ for 22-26 hours. To increase the appearance of metaphase, bisected embryos normally developed to the blastocyst were cultured in MOC-3 medium containing colcemid (0.04㎍/㎖) under the same condition for 3 hours. One of both demi-embryos was sexed by chromosomal analysis and the other was transferred to the uterine horn of a foster mother. The results obtained in these experiments were summarized as follows; 1. Of 241 pairs of demi-embryos cultured for 22-26 hours, 162 pairs (67.2%) were normally developed to the blastocysts. In the control groups, the percentage of intact and zona-free embryos normally developed to the blastocysts was 96.5% and 93.9%, respectively. 2. The mean Mitotic Index of 121 demi-embryos subjected to chromosomal analysis was 11.3±13.2%r and 18 demi-embryos (14.9%) were sexed. 3. Of 18 demi-embryos transferred to recipients after sexing, 2 offsprings were born and their sex was corresponded to the sex determined by chromosomal analysis.