Comparison of mechanical artificial shrinkage methods in mouse blastocyst vitrification = Comparison of mechanical artificial shrinkage methods in mouse blastocyst vitrification

국문 초록 (Abstract)

목적: This study was designed to determine which mechanical artificial shrinkage method, conducted by puncture, pipetting, or aspiration, was effective inincreasing the re-expansion rate of mouse blastocysts. 방법: In each group, 30 mouse blastocysts were used. Before vitrification, the blastocoelic cavity was collapsed by puncture with a micro-needle, pipetting with a micro-glass pipette, and direct aspiration with an ICSI pipette. After thawing, the re-expansion rate of blastocysts was examined for each artificial shrinkage method. Re-expansion rate was checked at 3, 5, and 7 hours after thawing. 결과: The number of re-expanded mouse blastocysts at 5 hours after thawing was12 in the puncture with a micro-needle group, 11 in the pipetting with a micro-glass pipette group, and 24 in the direct aspiration with an ICSI pipette group. The cumulative number of re-expanded mouse blastocysts at 7 hours after thawing was 20 in the puncture with a micro-needle group, 20 in the pipetting with a micro-glass pipette group, and 28 in the direct aspiration with an ICSI pipette group. There were statistically significant differences in the cumulative number of re-expanded mouse blastocysts between 5 hours and 7 hours after thawing (p=0.001 and 0.021, respectively). 결론: Direct aspiration with an ICSI pipette resulted in a higher re-expansion rate than the puncture and pipetting methods. It can be considered that the direct aspiration method is more convenient and simpler than the other two methods.