Effects of zinc oxide nanoparticle dispersants on cytotoxicity and cellular uptake

다국어 초록 (Multilingual Abstract)

Dispersion critically affects the physicochemical properties of nanoparticles and their interactions with biological systems. In this study, the effects of different zinc oxide nanoparticle (ZnO-NP) dispersants, that is, bovine serum albumin, citrate, carboxymethyl cellulose, fetal bovine serum, and cell culture medium, were investigated with respect to cytotoxicity and cellular uptake. Parallel comparative studies were also conducted with Zn2+ ions. The results demonstrated that ZnO-NPs dispersed in citrate exhibited the greatest cytotoxicity to human lung cells, probably related to their high cellular uptake via the citrate internalization mechanism, whereas, the energy-dependent endocytosis pathway of ZnO-NP internalization in cells was unaffected by dispersant type. These results emphasize that dispersant choice is important when evaluating the toxicity of nanoparticles and that results should be interpreted with caution.

참고문헌 (Reference)

1 Fan, Z., "Zinc oxide nanostructures: synthesis and properties" 5 : 1561-1573, 2005

2 Kachynski, A. V., "Zinc Oxide Nanocrystals for Non-resonant Nonlinear Optical Microscopy in Biology and Medicine" 112 : 10721-10724, 2008

3 Heng, B. C., "Toxicity of zinc oxide (ZnO) nanoparticles on human bronchial epithelial cells (BEA S-2B) is accentuated by oxidative stress" 48 : 1762-1766, 2010

4 Choi, S. J., "Toxicity evaluation of inorganic nanoparticles: considerations and challenges" 9 : 205-210, 2013

5 Chen, J. K., "The use of radioactive zinc oxide nanoparticles in determination of their tissue concentrations following intravenous administration in mice" 135 : 1742-1746, 2010

6 Kao, T. S., "Study of the optical response of phase-change recording layer with zinc oxide nanostructured thin film" 229 : 561-566, 2008

7 Leeuwenburgh, S. C. G., "Sodium citrate as an effective dispersant for the synthesis of inorganic-organic composites with a nanodispersed mineral phase" 6 : 836-844, 2010

8 Wells, M. A., "Serum proteins prevent aggregation of Fe2O3 and ZnO nanoparticles" 6 : 837-846, 2012

9 Song, W., "Role of the dissolved zinc ion and reactive oxygen species in cytotoxicity of ZnO nanoparticles" 199 : 389-397, 2010

10 Hirano, S., "Pulmonary clearance and toxicity of zinc oxide instilled into the rat lung" 63 : 336-342, 1989

11 Cho, S., "Precursor effects of citric acid and citrates on ZnO crystal formation" 25 : 3825-3831, 2009

12 Baek, M., "Pharmacokinetics, tissue distribution, and excretion of zinc oxide nanoparticles" 7 : 3081-3097, 2012

13 Bihari, P., "Optimized dispersion of nanoparticles for biological in vitro and in vivo studies" 5 : 14-, 2008

14 Djurišić, A. B., "Optical properties of ZnO nanostructures" 2 : 944-961, 2006

15 Paek, H. J., "Modulation of the pharmacokinetics of zinc oxide nanoparticles and their fates in vivo" 5 : 11416-11427, 2013

16 Madni, I., "Mixed surfactant system for stable suspension of multiwalled carbon nanotubes" 358 : 101-107, 2010

17 Sun, J., "Mitochondrial and plasma membrane citrate transporters: Discovery of selective Inhibitors and application to structure/function analysis" 2 : 101-110, 2010

18 Sharma, V., "Induction of oxidative stress, DNA damage and apoptosis in mouse liver after sub-acute oral exposure to zinc oxide nanoparticles" 745 : 84-91, 2012

19 Fernández, D., "In vitro evaluation of cellular responses induced by ZnO nanoparticles, zinc ions and bulk ZnO in fish cells" 452-453 : 262-274, 2013

20 Kim, J. S., "Evaluation of biocompatible dispersants for carbon nanotube toxicity tests" 85 : 1499-1508, 2011

21 Li, M., "Effects of water chemistry on the dissolution of ZnO nanoparticles and their toxicity to Escherichia coli" 173 : 97-102, 2013

22 Yu, J., "Effects of physicochemical properties of zinc oxide nanoparticles on cellular uptake" 304 : 012007-, 2011

23 Haniu, H., "Effect of dispersants of multi-walled carbon nanotubes on cellular uptake and biological responses" 6 : 3295-3307, 2011

24 Ji, Z., "Dispersion and stability optimization of TiO2 nanoparticles in cell culture media" 44 : 7309-7314, 2010

25 Heng, B. C., "Cytotoxicity of zinc oxide (ZnO)nanoparticles is influenced by cell density and culture format" 85 : 695-704, 2011

26 Cho, W. S., "Comparative absorption, distribution, and excretion of titanium dioxide and zinc oxide nanoparticles after repeated oral administration" 10 : 9-, 2013

27 Choi, S. J., "Biokinetics of zinc oxide nanoparticles: toxicokinetics, biological fates, and protein interaction" 9 (9): 261-269, 2014

28 Gao, L., "Acute toxicity of zinc oxide nanoparticles to the rat olfactory system after intranasal instillation" 33 : 1079-1088, 2013

29 Buford, M. C., "A comparison of dispersing media for various engineered carbon nanoparticles" 4 : 6-, 2007

30 Porter, D., "A biocompatible medium for nanoparticle dispersion" 2 : 144-154, 2008