Comparative carbohydrate metabolism in the shoots of a cold-hardy and a cold-sensitive peach (Prunus persica) cultivar during cold acclimation and deacclimation

다국어 초록 (Multilingual Abstract)

Carbohydrate metabolism affects cold hardiness in overwintering deciduous fruit trees. In this study, we compared thechanges in starch and soluble sugar contents, related enzyme activity, and gene expression in the shoots of ‘Soomee’ (SM)and ‘Odoroki’ (OD) peach (Prunus persica) trees during cold acclimation (CA) and deacclimation (DA). SM was coldhardierthan OD from December to March based on the LT 50 value, the temperature at which 50% injury occurred. Duringthis period, starch contents were significantly lower in SM than in OD, along with higher total amylase activity and relatedgene expression. In contrast, total soluble sugar contents were significantly higher in SM than in OD, exhibiting a closerelationship to cold hardiness. Of the detected soluble sugars, sucrose was predominant and its content was most significantlycorrelated with cold hardiness. Sucrose contents were significantly higher in SM than in OD from December to March.
Fructose and glucose contents in SM, but not in OD, increased during CA and then decreased during DA concurrently withincreased acid invertase activity and related gene expression. Raffinose and stachyose were detectable only from Novemberto April. Their contents and related synthase gene expression were also significantly correlated with cold hardiness, but werenot significantly different between the two cultivars. During CA, the activities of the enzymes involved in the synthesis ofsucrose, raffinose, and stachyose increased with the up-regulation of their related gene expression. During DA, in contrast,the activities of the enzymes involved in the degradation of starch, sucrose, raffinose, and stachyose increased with theup-regulation of their related gene expression. Furthermore, the differential cold hardiness in the shoots of different peachcultivars was associated with the difference in enzyme activities and gene expression, especially of acid invertase, regulatingsoluble sugar content during CA and DA.

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