Sulforaphene Attenuates Cutibacterium acnes -Induced Inflammation

다국어 초록 (Multilingual Abstract)

Acne is a chronic inflammatory disease of the sebaceous gland attached to the hair follicles.
Cutibacterium acnes is a major cause of inflammation caused by acne. It is well known that C. acnes secretes a lipolytic enzyme to break down lipids in sebum, and free fatty acids produced at this time accelerate the inflammatory reaction. There are several drugs used to treat acne; however, each one has various side effects. According to previous studies, sulforaphene (SFEN) has several functions associated with lipid metabolism, brain function, and antibacterial and anti-inflammatory activities. In this study, we examined the effects of SFEN on bacterial growth and inflammatory cytokine production induced by C. acnes. The results revealed that SFEN reduced the growth of C. acnes and inhibited proinflammatory cytokines in C. acnes-treated HaCaT keratinocytes through inhibiting NF-κB-related pathways. In addition, SFEN regulated the expression level of IL-1α, a representative pro-inflammatory cytokine expressed in co-cultured HaCaT keratinocytes and THP-1 monocytes induced by C. acnes. In conclusion, SFEN showed antibacterial activity against C. acnes and controlled the inflammatory response on keratinocytes and monocytes. This finding means that SFEN has potential as both a cosmetic material for acne prevention and a pharmaceutical material for acne treatment.

참고문헌 (Reference)

1 서대헌, "여드름의 약물요법" 대한의사협회 53 (53): 623-629, 2010

2 Zouboulis CC, "What is the pathogenesis of acne?" 14 : 143-152, 2005

3 Kow CS, "Use of sulforaphane in COVID-19 : Clinical trials are needed" 145 : 78-79, 2022

4 Gao L, "Traditional uses, phytochemistry, transformation of ingredients and pharmacology of the dried seeds of Raphanus sativus L. (Raphani Semen), A comprehensive review" 294 : 115387-, 2022

5 Zhang B, "Toll-like receptor 2 plays a critical role in pathogenesis of acne vulgaris" 3 : 4-, 2019

6 Andersen RK, "The social and psychological impact of acne treatment : A cross-sectional study of blood donors" 26 : 485-493, 2022

7 Tanghetti EA, "The role of inflammation in the pathology of acne" 6 : 27-35, 2013

8 Yang H, "Sulforaphene suppresses adipocyte differentiation via induction of post-translational degradation of CCAAT/enhancer binding protein Beta(C/EBPβ)" 12 : 758-, 2020

9 Zhang G, "Sulforaphene inhibits the progression of osteosarcoma via regulating FSTL1/NF-κB pathway" 263 : 118485-, 2020

10 Lim S, "Sulforaphene identified from radish (Raphanus sativus L.) seeds possesses antimicrobial properties against multidrug-resistant bacteria and methicillin-resistant Staphylococcus aureus" 24 : 131-141, 2016

11 Yang W, "Sulforaphene ameliorates neuroinflammation and hyperphosphorylated tau protein via regulating the PI3K/Akt/GSK-3β pathway in experimental models of Alzheimer's disease" 2020 : 4754195-, 2020

12 Dana AH, "Role of sulforaphane in endoplasmic reticulum homeostasis through regulation of the antioxidant response" 299 : 120554-, 2022

13 Kim J, "Review of the innate immune response in acne vulgaris : activation of Toll-like receptor 2 in acne triggers inflammatory cytokine responses" 211 : 193-198, 2005

14 Marron SE, "Quality-of-life research in acne vulgaris : current status and future directions" 20 : 527-538, 2019

15 Oh JH, "Purpurin suppresses atopic dermatitis via TNF-α/IFN-γ-induced inflammation in HaCaT cells" 36 : 3946320221111135-, 2022

16 Chen Q, "Propionibacterium acnes-induced IL-8 production may be mediated by NF-κB activation in human monocytes" 29 : 97-103, 2002

17 Beylot C, "Propionibacterium acnes : an update on its role in the pathogenesis of acne" 28 : 271-278, 2014

18 Wang L, "Preparation of sulforaphene from radish seed extracts with recombinant food-grade Yarrowia lipolytica harboring high myrosinase activity" 69 : 5363-5371, 2021

19 Valente Duarte De Sousa IC, "New and emerging drugs for the treatment of acne vulgaris in adolescents" 20 : 1009-1024, 2019

20 Hernandez-Quintero M, "Interleukin-6 promotes human epidermal keratinocyte proliferation and keratin cytoskeleton reorganization in culture" 325 : 77-90, 2006

21 Kistowska M, "IL-1beta drives inflammatory responses to propionibacterium acnes in vitro and in vivo" 134 : 677-685, 2014

22 Zaenglein AL, "Guidelines of care for the management of acne vulgaris" 74 : 945-973, 2016

23 Jayasinghe AMK, "Fucoidan Isolated from Sargassum confusum suppresses inflammatory responses and oxidative stress in TNF-α/IFN-γ-stimulated HaCaT keratinocytes by activating Nrf2/HO-1 signaling pathway" 20 : 117-, 2022

24 Gao L, "Examination of the differences between sulforaphane and sulforaphene in colon cancer : A study based on next-generation sequencing" 22 : 690-, 2021

25 Heo Y, "Development of skin sensitization alternative test through co-culture of THP-1 dendritic cell line and HaCaT keratinocyte cell line" 7 : 29-34, 2013

26 Temiz SA, "Dapsone for the treatment of acne vulgaris : do the risks outweigh the benefits?" 41 : 60-66, 2022

27 Dixit N, "Chemokines, selectins and intracellular calcium flux : temporal and spatial cues for leukocyte arrest" 3 : 188-, 2012

28 Zheng W, "Biological mechanisms and clinical efficacy of sulforaphane for mental disorders" 35 : e100700-, 2022

29 Fang F, "Baicalin suppresses Propionibacterium acnes-induced skin inflammation by downregulating the NF-κB/MAPK signaling pathway and inhibiting activation of NLRP3 inflammasome" 53 : e9949-, 2020

30 Kim J, "Activation of toll-like receptor 2 in acne triggers inflammatory cytokine responses" 169 : 1535-1541, 2002

31 Hywel C Williams, "Acne vulgaris" Elsevier BV 379 (379): 361-372, 2012

32 Mohsin N, "Acne treatment review and future perspectives" 35 : e15719-, 2022

33 Boen M, "A Review and update of treatment options using the acne scar classification system" 45 : 411-422, 2019