인삼 사포닌이 개 심실 형질막의 K+-의존성 포스파타제 활성에 미치는 영향 = Effect of Ginseng Saponins on K+-Dependent Phosphatase Activity of Dog Cardiac Sarcolemma

다국어 초록 (Multilingual Abstract)

The effects of ginseng saponins, gypsophila saponin, sodium dodecyl sulfate(SDS), and Triton X-100 on membrane K^(+)-dependent phosphatase activity which is lipid dependent and represents dephosphorylation step of the complete Na^(+), K^(+)-ATPase reaction were investigated in this study to elucidate whether the effects of ginseng saponins are due to the detergent action, using sarcolemma enriched preparation isolated from dog ventricle. Na^(+), K^(+)-ATPase and K^(+)-dependent phosphatase activities of cardiac sarcolemma were about 143 μmol Pi/mg protein/hr and 34 μmol p-nitrophenol/mg protein/hr, respectively. While ginseng saponins (triol＞total＞diol) inhibited K^(+)-dependent phosphatase activity, gypsophila saponin, and low dose of SDS(0.4 μg/μg protein), and Triton X-100 (0.6 μg/μg protein) increased the enzyme activity, indicating disruptive effect of detergents on membrane barriers. The activating effect of low doses of Triton X-100 on membrane K^(+)-dependent phosphatase appeared at concentration decreasing light scattering. However, the inhibitory effect of ginseng saponin appeared before a decrease in light scattering. These results suggest that low concentrations of ginseng saponins inhibit the membrane K^(+)-dependent phosphatase by interacting directly with enzyme before membrane disruption.