RISS 검색 - 학위논문 상세보기

다국어 초록 (Multilingual Abstract)

In biological systems, numerous biological components work with complexed pathway, but a series of procedures of reaction cascade are regulated in a sophisticated manner. As the key components involved in sophisticated regulation are discovered, many researchers have published their studies about engineering application of the biological system. In this work, among the various biological reactions, we focused on identification strategy of selective coupling reactions between biological components for biosensor applications. In order for identification of reaction signal, we used polydiacetylene (PDA) nanomaterial and gold nanoparticle (AuNP) which has unique optical properties. This thesis divided to four chapters. In first chapter, we described research background and, especially introduced physical and optical properties of nanomaterials to aid understanding of this study. In second chapter, we described several identification results for engineering application that based on PDA nanomaterial. We developed hydrogel encapsulated PDA based genetically modified (GM) crop detection sensor and fish-gill like microfluidic channel embedded PDA sensor for bacteria detection, and PDA functionalized paper-based toxin, bacterial spore, detection kit. In third chapter, we introduced AuNP based identification result and engineering applications. We developed AuNP coated plastic chip based portable GM crop detection system that utilize localized surface plasmon resonance (LSPR) phenomenon of AuNP, and we introduced simplified bioreceptor evaluation kit based on AuNP embedded lateral flow immunoassay (LFIA) strip. In forth chapter, we summarized this work and overlooked future research. Through this study, we could be able to identify invisible biological coupling interactions for engineering applications. We expected that the detection and evaluation platform that developed in this study for specific purpose could be able to apply in practical application such as the point-of-care testing (POCT) platform.

번역하기

목차 (Table of Contents)

1. CHAPTER 1. INTRODUCTION 1
1.1. Introduction 2
Identification of Coupling interaction between biological components and their biosensor applications 2
Nanomaterial assisted identification of coupling interaction between biological components 3
Polydiacetylene (PDA) nanomaterialbased identification 4

1. CHAPTER 1. INTRODUCTION 1
1.1. Introduction 2
Identification of Coupling interaction between biological components and their biosensor applications 2
Nanomaterial assisted identification of coupling interaction between biological components 3
Polydiacetylene (PDA) nanomaterialbased identification 4
Gold nanoparticle (AuNP)based identification6
1.2. Organization of dissertation 8
2. CHAPTER 2. Polydiacetylene (PDA) based Platform 14
2.1. Chromatic Biosensor for Detection of Phosphinothricin Acetyltransferase by Use of Polydiacetylene Vesicles Encapsulated within Automatically Generated Immunohydrogel Beads 15
Introduction 15
Materials and methods 18
Results and discussion 23
Conclusions 35
2.2. An antibacterial microfluidic system with fish gill structure for the detection of Staphylococcus via enzymatic reaction on a chromatic polydiacetylene material caused by lysostaphin 36
Introduction 36
Materials and methods 38
Results and discussion 42
Conclusions 51
2.3. AptamerConjugated Polydiacetylene Colorimetric Paper Chip for the Detection of Bacillus thuringiensis Spores 52
Introduction 52
Materials and methods 56
Results and discussion 59
Conclusions 68
3. CHAPTER 3. Gold nanoparticle (AuNP) based Platform 69
3.1. Identification of genetically modified DNA found in Roundup Ready soybean using gold nanoparticles 70
Introduction 70
Materials and methods 72
Results and discussion 74
Conclusions 83
3.2. Receptor Norovirus Targeted Bioreceptor Screening Method based on Lateral Flow Immunoassay (LFIA) 84
Introduction 84
Materials and methods 86
Results and discussion 91
Conclusions105
4. CHAPTER 4. CONCLUSION AND FUTURE WOTKS 106
4.1. Conclusions 107
4.2. Future Works 110
5. REFERENCES 111

참고문헌 (Reference)

1 Laurent, P., Wilson, J. M., "Fish gill morphology: Inside out", In pp. 192–213, 2002

2 Ahn, D. J., Yang, D. H., Lee, Y. B., Lee, J. S., Lee, G. S., Kim, J. M., "A polydiacetylene-based fluorescent sensor chip", 127, 17580–17581 https://doi. org/10.1021/ja0547275, 2005

3 Fernandez, T., "Detecting Genetically Modified Products in Food", 72, 454A-459A, 2000

4 Byrne, H., Sharma, S., O’Kennedy, R. J., "Antibodies and antibody-derived analytical biosensors", 60, 9–18 https://doi. org/10.1042/EBC20150002, 2016

5 Mahmood T, Yang PC, "Western blot: technique, theory, and trouble shooting", 4, 429–434 https://doi. org/10.4103/1947-2714.100998, 2012

6 Jiang, L., Su, Y. L., Li, J. R., "Chromatic immunoassay based on polydiacetylene vesicles", 38, 29–33 https://doi. org/10.1016/j. colsurfb.2004.08.010, 2004

7 Su, Y. L., "Assembly of polydiacetylene vesicles on solid substrates", 292, 271–276 https://doi. org/10.1016/j. jcis.2005.05.049, 2005

8 Cheun, H. I., Watarai, M., Uchida, I., Takeshi, K., Makino, S. I., "Detection of anthrax spores from the air by real-time PCR", 33, 237–240 https://doi. org/10.1046/j.1472-765x.2001.00989. x, 2001

9 Kerby, M. B., Tripathi, A., Legge, R. S., "Measurements of kinetic parameters in a microfluidic reactor", 78, 8273–8280 https://doi. org/10.1021/ac061189l, 2006

10 Siegel, J. P., "The mammalian safety of Bacillus thuringiensis-based insecticides", 77, 13–21 https://doi. org/10.1006/jipa.2000.5000, 2001