인삼이 사람태아골모세포의 세포주기조절에 미치는 영향 = Effects of Ginseng Radix on the Cell Cycle Regulation in Human Fetal Osteoblast

다국어 초록 (Multilingual Abstract)

Ginseng Radix(GR) has been used widely as oriental medicine and the effects of it have been investigated by many researchers. The purpose of present study was to investigate the effects of GR on the cell cycle progression and its molecular mechanism in human fetal osteoblast. The result s were as follows.
Increased cell proliferation was observed in cells exposed to 100ng/ml, 10ng/ml of GR-1 at 12 hours and 24 hours, 1㎍/㎖ of GR-1 at 48 hours, and 100㎍/㎖, 10㎍/㎖ of GR-2 at 12 hours, all treatment groups of GR-2 at 24 hours(p<0.05).
S phase and G1 phase was increased in the group of treated with 100ng/ml Of GR-1, with 10㎍/㎖ and 1㎍/㎖ of OR-2, with 100㎍/㎖ and 10㎍/㎖ of GR-3 in the cell cycle analysis.
The cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4 and CDK 6 were increasad in the group of treated with 1㎍/㎖ and 100ng/ml of GR-1, with 10㎍/㎖ and 1㎍/㎖ Of GR-2, with 100㎍/㎖ and 10㎍/㎖ of GR-3.
On the other hand, p21 was decreased in the treatment group with 1㎍/㎖ and 100 ng/㎖ of GR-1, with 10㎍/㎖ and 1㎍/㎖ Of GR-2, 10㎍/㎖ Of GR-3, and p53 and p16 was decreased in the treatment group with 100ng/mll Of GR-1, 100㎍/㎖ GR-3 and pRb W ~ S decreased in the all treatment group except 1㎍/㎖ of GR-1.
These results suggested that GR increases the cell proliferation and the cell cycle progression in human fetal osteoblast, which is linked to increased cell cycle regulation protein levels of Cyclin D1, Cyclin E, CDK 2, CDK 4, CDK 6 and decreased ell cycle regulation protein levels of p21, pRb.

목차 (Table of Contents)

• Ⅰ. 서론
• Ⅱ. 연구 대상 및 방법
• Ⅲ. 연구 성적
• Ⅳ. 총괄 및 고찰
• Ⅴ. 결론
• 참고문헌
• ABSTRACT