Antioxidant and Lifespan Extending Property of Quercetin-3-Odirhamnoside from Curcuma longa L. in Caenorhabditis elegans

다국어 초록 (Multilingual Abstract)

Quercetin-3-O-α-L-rhamnopyranosyl(1→2)-O-α-L-rhamnopyranoside(QDR) was isolated from the remaining undergroundparts of Curcuma longa after harvesting the medicinal parts, andthe antioxidant activities in vitro and lifespan-extending effect ofQDR were elucidated using the Caenorhabditis elegans modelsystem. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicalscavenging effect of QDR showed similar potent activities incomparison with vitamin C. QDR also showed strong superoxidequenching activities as measured by the riboflavin- and xanthineoriginatedsuperoxide quenching activities. QDR demonstratedpotent lifespan extension of worms under normal culturecondition. Subsequently, the protective effect of QDR on the stressconditions such as thermal and oxidative stresses was determined.
In the case of heat stress, QDR-treated worms exhibited enhancedsurvival rate, as compared to control worms. In addition, QDR-fedworms lived longer than control worms under oxidative stressinduced by paraquat. To verify the possible mechanism of QDRmediatedincreased lifespan and stress resistance of worms, weinvestigated whether QDR might alter superoxide dismutase(SOD) activity and intracellular reactive oxygen species (ROS)levels. Our results showed that QDR was able to elevate SODactivity of worms and reduce intracellular ROS accumulation in adose-dependent manner.

참고문헌 (Reference)

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