Immunoblot와 면역황금 표지법을 이용한 선모충 특이항원의 분석 = The Characterization and Localization of antigenic protein of Trichinella spiralis larvae

다국어 초록 (Multilingual Abstract)

The newborn Trichinella spiralis larvae is capable of penetrating and infecting striated and skeletal muscle fibers of most mammalian species. The parasites often can not be detected by direct microscopical examination.
Serodiagnosis may helpful in such diagnostic difficulties. However, any serological method for the diagnosis of trichinellosis has not yet been available on account of its cross reactivity. The present study was performed to observe the specifically reacting antigenic bands of gel filtration antigen of the crude extract Trichinella larval worm and their reaction to the serum IgG antibody obtained from experimentally immunized and infected rats. The localization of specific and cross reacting antigenic bands were also observed by immunogold labeling method.
The results obtained were as follows:
1. Serum leveles of IgG antibody began to increase from 6 day after infection.
2. SDS-PAGE profile showed that each antigen consisted of more than 20 polypeptides of molecular mass 45-220 kDa in F1, 45-180 kDa in F2, 29-200 kDa in F3, 13-120 kDa in F4, 14-80 kDa in F5, 14-55 kDa in F6, and below 20 kDa in F7
3. Major antigenic bands which reacted to specific IgG antibody infected with Trichinella larvae were 16, 45, 55 and 60 kDa band. A 45 kDa band was identified as common antigen band.
4. Gold particles were labeled on the cuticle, hypodermis surface, esophagus-occupying substance, midgut-occupying substance and α-granule in stichocyte.
5. In the tissue reacted to serum of rabbit immunized with 45 kDa protein as common band, gold particles were labled on the cuticle surface and α,β-granules in stichoyte. Electron micrographs of tissue reacted to rabbit serum immunized with 60 kDa protein, which was considered as specific antigen band, showed that gold particles were labeled on the hypodermis, esophagus-and midgut-occupying substance and α-granules in stichocyte.