Comparison of Biological Activities on Extracts and Fractions in Compositae Plants

국문 초록 (Abstract)

본 연구는 우리나라 전역에 자생하는 국화류3종 학슬, 희렴, 한련초를 대상으로 MeOH 80% 추출물 및 헥산, 클로 로포름, 에틸아세테이트, 부탄올 및 물분획을 이용한 항균활성, 항산화 및 tyrosinase 저해활성효과에 대한 연구를 통해 기능성 화장품소재로서의 이용가능성을 확인하고자 하였다. 연구결과 항균활성의 경우 MeOH 80% 추출물에서는 한련초 와 용매분획 중에서는 학슬의 헥산분획, 희렴의 클로로포름분획, 에틸아세테이트 분획, 한련초의 클로로포름분획을 중심 으로 항균력을 나타내었다. 항산화 활성의 경우 3종 모두 용매분획 중 에틸아세테이트, 부탄올 분획에서 90%이상의 효과 를 나타내었다. Tyrosinase 저해활성의 경우 희렴과 학슬의 에틸아세테이트 분획물에서 우수한 활성을 나타내었다. 이상 의 국화류3종 MeOH 80%추출물 및 분획물에서 항산화 및 미백작용이 우수하므로 기능성화장품소재 개발 활용이 가능할 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

This study was conducted to investigate the anti-oxidative, anti-oxidative and tyrosinase inhibitory effects of MeOH 80% extract and hexane, chloroform, ethyl acetate, butanol and aqueous fraction on three kinds of compositae plants in Korea. In the antimicrobial effect, the extract and chloroform fraction of Eclipta prostrata and hexane fraction of Carpesium abrotanoides L. and chloroform fraction of Siegesbeckia glabrescens exhibited significant inhibition. The antioxidant activity of ethyl acetate and butanol fractions was more than 90% in all three plants. In case of tyrosinase activity, showed a potent inhibition ethlyacetate fraction of Siegesbeckia glabrescens and Carpesium abrotanoides L, which were higher than control group. In MeOH 80% extracts, there was not found to have antimicrobial, anti-oxidant and tyrosinase inhibitory activity, however there was ethylacetate fraction of Siegesbeckia glabrescens to show effectss commonly in the three assay system.

목차 (Table of Contents)

• Abstract
• 요약
• 1. Introduction
• 2. Materials and methods
• 2.1 Production of experimental material
• 2.2 Preparation of Extracts and Fractions
• 2.3 Antimicrobial activity of extracts and fractions against Staphylococcus aureus
• 2.4 Antimicrobial activity of extracts and fractions against Pseudomonas aeruginosa
• 2.5 Antimicrobial activity of extracts and fractions against Escherichia coli
• 2.6 Antimicrobial activity of extracts and fractions against Aspergillus niger
• 2.7 Antioxidative activity by DPPH assay
• 2.8. Inhibition of tyrosinase activity of extracts and fractions
• 3. Results and Discussion
• 3.1 Antimicrobial activity of extracts and fractions
• 3.2 Antioxidative effect of extracts and fractions
• 3.3 tyrosinase inhibitory effect of extracts and fractions
• 4. Conclusion
• REFERENCES