Vitamin D3와 Dexamethasone의 복합 투여가 골모세포에 미치는 영향에 관한 연구 = The Effect of Admixture of Vitamin D3 and Dexamethasone on Osteoblastic Cells

다국어 초록 (Multilingual Abstract)

Bone is a dynamic tissue which is constantly remodelled by subsequent cycles of bone resorption and formation. Glucocorticoid and vitamin D_3 are known as regulating substances in bone metabolism. In vitro experiments using bone tissue, it was suggested that glucocorticoid inhibits bone resorption, whereas the effect of glucocorticoid on bone formation are complex- increasing or decreasing effect. The active form of vitamin D_3. 1,25-dihydroxycholecalciferol[I.25-(OH)_2D_3], has been reported to stimulate osteoblastic activities including the production of ALP, type I collagen, and osteoclacin.
The purpose of this study was to evaluate the effect of admixture of vitamin D_3 and dexamethasone, one of glucocorticoids, on osteoblastic cell line(MC3T3-El). Alkaline phosphatase(ALP) and MTT assay were conducted in the cultivated cells with 1, 10, 100nM/㎖ of 1,25-(OH)_2D_3 and/or 1μM/㎖, 100μM/㎖, 10nM/㎖ dexamethasone.
The observed results were as follows.
1. The activity of osteoblastic cells with 1μM/㎖ of dexamethasone was significantly increased at 1-day cultivation with comparison to control group, but was decreased afterwards. But the activity of ALP was greatest in 1μM/㎖ of dexamethasone and increased wth time lapsed.
2. The activity of osteoblastic cells with vitamin D_3 was significantly increased dose-dependently at I-day cultivation, but was significantly decreased in 100nMJ/.㎖ at 2-day cultivation, and was a little increased again at 3-day cultivation. The activity of ALP was increased in 10nM/㎖ or l11nM/㎖ at 2-day or 3-day cultivation, and was greatest in l00nM/㎖ at 3-day cultivation.
3. In case of admixture of dexamethasone and vitamin D_3, the cellular activity was decreased in any concentration of vitamin D_3 at 2-day cultivation, but was increased again at 3-day cultivation, which was greater than that in control or dexamethasone only group. The activity of ALP was decreased at 1-day cultivation, but was increased in the admixture of 10nM/㎖ or 100nM/㎖ of dexamethasone with l00nM/㎖ of vitamin D_3 at 2-day cultivation, and was again decreased at 3-day cultivation