동결융해 후 토끼 정자의 Matrix Metalloproteinases (MMPs)와 Their Tissue Inhibitors (TIMPs) 발현 양상

다국어 초록 (Multilingual Abstract)

We observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. According to the results from each freezing extender, the sperm membrane integrity (HOST: Hypoosmotic Swelling Test) analysis in TCGGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Glycerol 3%, Dimethylsulpoxide 3.5 M) is 59.8 ± 0.7, TCGSD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Sucrose 0.1 M, Dimethylsulpoxide 3.5 M) is 59.3 ± 0.5 were significantly higher (p < 0.05) among the experimental groups. And MMPs analysis result, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of active MMP-2 was the highest in sperms frozen in TCGSD and TCGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Dimethylsulpoxide 3.5 M), Meanwhile, sperms from the TCGGD and TCGED (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Ethylene glycol 3%, Dimethylsulpoxide 3.5 M) group showed lower level of active MMP-2 expression. Together, these results indicate that adding glycerol or sucrose to the sperm freezing buffer would not only suppress MMPs expression but also minimize DNA fragmentation, providing a mean to improve the success rate in the in vitro manipulation of rabbit sperms. Therefore, these results suggest that TCGGD or TCGSD extender method for freezing-thawing of rabbit sperm increased the viability after thawing.

목차 (Table of Contents)

• ABSTRACT
• 서론
• 재료 및 방법
• 정액 채취
• 동결 희석액
• 정액 동결 및 동결 정액 융해
• 원형질막 기능 검사
• 동결 정자의 해동 이후 DNA 절편분석
• MMP-2, MMP-9의 효소활성화 분석
• MMP-2, MMP-9 및 inhibitor의 단백질 발현양상
• 통계 분석
• 결과
• 동결보호제에 따른 토끼 정자의 원형질막 정상성 분석
• 동결보호제에 따른 DNA 손상도 분석
• 동결보존액에 따른 세포외 기저질 분해효소의 활성 및 비활성도 분석
• 동결보존액에 따른 MMPs와 TIMPs의 발현양상
• 고찰
• CONFLICTS OF INTEREST
• ORCID
• REFERENCES