Synthesis of Molecularly Imprinted Polymer (MIP) by Radiation-Induced Polymerization and Separation of Ferulic Acid from Rice Oil Using Mip-packed

다국어 초록 (Multilingual Abstract)

A molecularly imprinted polymer (MIP) was synthesized by radiation-induced polymerization (RIP),
where the ferulic acid was used as a template molecule, 4-vinylpyridine as a monomer and ethylene glycoldimethacrylate
(EGDMA) as a cross-linking monomer. The MIP was packed in a glass column using a slurry
method for use in medium pressure liquid chromatography (MPLC). The MPLC column was tested for separation
and purification of ferulic acid from the rice oil. When repeated three times, the MPLC separation/purification
yielded the ferulic acid with the purity higher than ~99%. The chemiluminescence of the luminal (5-amino-2,3-
dihydro-1,4-phtalazinedione) measured on a potato disc slide (5.0 mm thick) was enhanced in the presence of
ferulic acid, while, without the ferulic acid, the chemiluminescence of luminol on the potato slice disc was not
observed, which suggests the ferulic acid obtained from the rice oil can be useful for immunoassay.

참고문헌 (Reference)

1 Wolf, F. T, "Pflanzenphysiol." 80 : 243-, 1976

2 Bartsch, R. A, "Molecular and Ionic Recognition with Imprinted Polymers" American Chemical Society, Washington DC 1997

3 Yoshida, H, "Molecular Plant-Microbe Interactions" 14 (14): 725-736, 2001

4 Anderson, L. I, "Molecular Interaction in Bioseparation" Plenum Press, New York 383-, 1993

5 Komiyama, M, "Molecular Imprinting" Wiely, Mörlenbach, Germany 2003

6 Muller, R, "Makrornol. Chem. Rapid Commun." 14 : 637-, 1993

7 Choi, S.-H, "J. Appl., Polym. Sci." 88 (88): 1153-1161, 2003

8 Kang, Y.-O, "J. Appl. Polym. Sci." 100 (100): 1809-1815, 2005

9 Choi, S.-H, "J. Appl. Polym. Sci." 91 (91): 2335-2342, 2004

10 Shea, K.J, "J. Amer. Chem. Soc." 115 : 3368-3369, 1993

11 Stott, R. A. W, "Clin. Chem." 32 : 1073-, 1986

12 Hosoda, A, "Biorg. Med. Chem." 10 : 1189-1196, 2002

13 Lundin, A, "Bioluminescence and Chemiluminescence" New Perspectives, Wiley, Chichester 555-, 1987

14 Thorpe, G. H. G, "Bioluminescence and Chemiluminescence" New Perspectives Wiley, Chichester 199-, 1987

15 Taniguchi, H, "Anticancer Res." 19 : 3757-3762, 1999

16 Wulff, G, "Angew. Chem. Int. Ed. Engl." 11 : 341-, 1972

17 Piletska, E. V, "Analytica Chimica Acta." 542 : 111-117, 2005

18 Sellergren, B, "Analy. Chem." 66 : 1578-, 1994

19 Yoon, Seok-Kee, "Anal. Sci. Tech."

20 Sánchez, F. G, "Anal. Chem. Acta" 310 : 399-406, 1995

21 Beach, J. V, "Amer. Chem. Sot." 116 : 379-, 1994

22 Ansell, R. J, "Advanced Drug Delivery Reviews" 57 : 1809-1835, 2005