The Chemotaxonomic Relationship of Vibrio cholerae non-O1 by Fatty Acid Compositions

국문 초록 (Abstract)

V. cholerae non-O1 49균주와 V. cholerae O1, V. mimicus, V. vulnificus와 V. parahaemolyticus의 균체지방산(fatty acid methyl ester; FAME)을 gas liquid chromatogrphy로 분석하였다. 이들 분석자료를 통계학적으로 처리하여 Vibrio 종과 V. cholerae의 혈청형별 유연성을 비교 검토하였다. 검출된 지방산은 모두 41종이었고 분포량이 많은 것은 16:0, 16:1 cis 9, 18:1 trans 9/16/cis 11과 15:0 iso 2 OH/16:1 cis 9 였다. 검출된 지방산 중에서도 35종은 V. cholerae를 동정하는데 주요한 인자로 작용되었다. 지방산분포를 UPGMA(비가중수리분석)으로 dendrogram을 작성한 결과 V. cholerae non-O1은 V cholerae O1보다 V mimieus가 더 높은 유사도를 나타내었다. 특히 hydoxy acid는 V. chozerae의 아종단위를 동정하는데 중요하였다. V. choEeore non-O1중에서 O2, O5, O8, O10, O14, O27, O37, O39, O45와 O69의 총 10 종류 혈청형을 대상으로 지방산 조성에 의 한 유사성을 검토한 결과 유사도가 92% 이상 수준에서 7개의 아종를 형성하여 혈청형과 지방산 조성간에는 유의할 만한 상관관계가 있었다. 따라서 V. cholerne non-O1의 동정 및 역학적인 조사시 지방산 분석은 유용하게 활용될 수 있음을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

The authors attempted utilization of fatty acid composition of vibrios as a tool for identification of the strains. Fatty acid of 49 strains of Vibrio cholerae non-01, V. cholerae 01, V. mimicus, V. vulinificus and V. parahaemolyticus was analyzed by gas-liquid chromatography column. According to the statistical analysis of the fatty acid data, the relationship between the Vibrio species and serotypes of the strains was discussed. Forty one kinds of fatty acid were de- tected from the tested strains and 35 kinds of fatty acids among the detected fatty acids were significant factors to identify the vibrios. The predominant fatty acids were 16:0, 16:l cis 9, 18:l trans 9/6/cis 11 and 150 is0 20H/16:1 cis 9 as above about 20% in total. Fatty acid compositions of the Vibrio species were an important factor in identifylng their subspecies either predominant fatty acids or minor ones. According to the analysed results by a conventional statistical pro- cessing method (UPGMA) and prepared dendrogram, V. cholerae non-01 had more closer re- lationship with v. mimicus compared with v. cholerae 01. Moreover, the distribution of hydroxy acid was a significant factor for identifying V. cholerae subspecies. Comprising all the 10 sero- types detected from V. cholerae non-01 examined such as 0 2 , 05, 08, 010, 014, 027, 037, 039, 045 and 069, we could group them into seven subspecies by cluster analysis with the similarity value of fatty acid composition as above 92%. It means that there is a significant relationship between serotypes and fatty acid composition of V . cholerae. These results indicated that num- erical analysis of fatty acid composition data of V. cholerae non-01 could classify them into sub- species, and also which may provide a useful epidemiologic information or a basis for further analysis such as PCR and DNA probe analysis.