두툽상어 ( Scyliorhinus torazame ) Metallothionein cDNA 의 cloning 및 이의 분자적 특성 = Molecular Cloning and Characterization of a Novel Metallothionein Isoform Expressed in Tiger Shark ( Scyliorhinus torazame )

다국어 초록 (Multilingual Abstract)

A complementary DNA encoding metallothionein(MT), a heavy metal-responsive protein was cloned from a cartilaginous shark species. Scyliorhinus torazame. An expressed sequence tag(EST)from the shark liver, which showed high similarity with a MT gene, was isolated and its full-length sequence(390bp)was determined. The putative shark MT cDNA sequence contained an open reading frame consisting 68 amino acids and 182bp of 3-untranslated region including the poly (A+) signal. The deduced amino acid sequence was 41-54% identical to those of other animals including mammals and fish species. Tiger shark MT cDNA showed high conservation in the Cys regions. however, peculiarly contained not only additional five amino acids just prior to the conserved beta-domain but also a Ser residue at C terminal, which has not been seen in other MT sequences.