양돈장 살모넬라 혈청 양성율과 도축돈 살모넬라균 분리율의 상관관계 및 분리 균주의 항생제 내성 = Correlation between the Salmonella seroprevalence on farms and the isolation rate from slaughtered pigs and antimicrobial resistance from the isolates

다국어 초록 (Multilingual Abstract)

Salmonella spp. are one of the most common bacteria that causes heavy losses in swine industry and have implications for public health. In this study, the correlation between Salmonella seroprev-alence on farms and the isolation rate from slaughtered pigs was analyzed and the antimicrobial resistance of the isolated Salmonella spp. was investigated. A total of 3,001 serum samples for ELISA were collected from 17 farms during two consecutive years (2012-2013). The mean values of ELISA OD% for each 8 age groups were as follows; gilt 27.83 (n=472), sow 23.75 (n=367), 150 days (d) of pig 16.53 (n=278), 130 d 11.87 (n=366), 100 d 9.46 (n=378), 20 d 9.17 (n=394), 70 d 6.56 (n=382), 40 d 3.72 (n=364). From July 2013 to January 2014, a total of 53 (8.0%) Salmonella strains were isolated from 665 slaughtered pigs shipped from those 17 farms. The mean values of ELISA OD% for each age groups serum samples that were collected in the second half of 2013 showed a positive correlation at 100 d (0.61, P＜0.05), 130 d (0.45, P＜0.1) with the isolation rate of Salmonella spp. in the slaughtered pigs. All the isolates were identified by a real-time PCR and tested for antimicrobial susceptibility. As a result, the predominant serovar was S. Typhimurium (52.8%) and there were 15 strains showing their own antimicrobial resistance pattern. All strains were susceptible to amoxicillin, cefepime, ciprofloxacin and some of them were resistant to streptomycin and tetracycline (60%), ampicillin (53.3%), chloramphenicol (33.3%), respectively.

참고문헌 (Reference)

1 국립수의과학검역원, "동물질병 표준검사법"

2 하도윤, "도축장의 소와 돼지 분변에서 분리한 살모넬라속의약제내성 및 약제내성 유전자의 보유율" 한국가축위생학회 34 (34): 45-54, 2011

3 최원종, "도축돈에서 분리된 살모넬라의 혈청형 및 유전형" 한국가축위생학회 31 (31): 1-16, 2008

4 Raul CM, "Survey on Salmonella prevalence in slaughter pigs from Saskatchewan" 49 : 793-796, 2008

5 Hokyoung Jung, "Serovars distribution and antimicrobial resistance patterns of Salmonella spp. isolated from the swine farms and slaughter houses" 대한수의학회 51 (51): 123-128, 2011

6 김영훈, "Seroprevalence of Swine Salmonellosis in Korean Swine Herds" 한국축산식품학회 30 (30): 62-65, 2010

7 Rostagno MH, "Salmonella prevalence in market-age turkeys on-farm and at slaughter" 85 : 1838-1842, 2006

8 Kim JY, "Salmonella prev-alence in market weight pigs before and after shipment to slaughter ISECSP" 139 : 1999

9 APHIS, "Salmonella on U.S. swine sites-prevalence and antimicrobial susceptibility"

10 Steinbach G, "Salmonella infections in swine herds-epidemiology and importance for human diseases" 106 : 282-288, 1999

11 Futagawa-Saito K, "Salmonella in healthy pigs : Prevalence, serotype diversity and antimicrobial resistance observed during 1998-1999 and 2004-2005 in Japan" 136 : 1118-1123, 2008

12 Hurd HS, "Salmonella enterica infections in market swine with and without transport and holding" 68 : 2376-2381, 2002

13 EFSA, "Risk assesment and mitigation options of Salmonella in pig production" 4 : 1-131, 2006

14 EFSA, "Report of the Task Force on Zoonoses Data Collection on the analysis of the baseline survey on the prevalence of Salmonella in slaughter pigs" 135 : 1-111, 2008

15 Lee WW, "Prevalence and antimicrobial susceptibility of Salmonella isolated from Korean slaughter pigs" 26 : 313-321, 2003

16 박봉균, "Prevalence and antimicrobial resistance patterns of Salmonella spp. isolated from different aged pigs in Korea" 대한수의학회 47 (47): 395-398, 2007

17 Kim HB, "Prevalence and antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from pigs at slaughterhouses in Korea" 5 : 823-830, 2011

18 CLSI, "Performance standards for antimicrobial susceptibility testing; Twenty-fourth informational supplemen"

19 Larsen ST, "Impact of commercial preharvest transportation and holding on the prevalence of Salmonella enterica in cull sows" 66 : 1134-1138, 2003

20 Steven AC, "Disease of Swine" John Wiley & Sons 821-833, 2012

21 MAFF, "Code of practice for the prevention and control of Salmonella on pig farms"

22 Bahnson PB, "Associations between on-farm and slaughter plant detection of Salmonella in market-weight pigs" 68 : 246-250, 2005

23 Nielsen B, "A new Salmonella surveillance and control programme in Danish pig herds and slaughterhouses" 114 : 323-326, 2001

24 농림축산식품부, "2012년도 축산 항생제내성균 감시체계구축 보고서" 2013

25 김수란, "2010년 가축 및 도축장 식육에서 분리한 salmonella의 항생제 내성" 한국예방수의학회 35 (35): 246-254, 2011