Screening method for the isolation of anticancer molecule from microbial extract was entablished by using sulforhodamine B(SRB) assay, Hep-2 and PC14 cell lines which were derived from human epidermoid carcinoma and prostate adenocarcinoma respectivel...
Screening method for the isolation of anticancer molecule from microbial extract was entablished by using sulforhodamine B(SRB) assay, Hep-2 and PC14 cell lines which were derived from human epidermoid carcinoma and prostate adenocarcinoma respectively. The effect of cell number on SRB assay showed the absorbance is proportional to cell number. The growth rates of cell lines were determined using the SRB assay. These results can be used for the optimization of screening test. Primary screening test showed that extracts of Actinomycetes isolated from soil have cytotoxic activity to PC14 and Hep-2 cell lines.