규칙적인 운동이 고혈압유발 흰쥐의 심근에서 Bcl-2발현 및 Apoptosis변화에 미치는 영향 = The Effects of Regular Exercise on the Expression of Bcl-2 and Apoptosis in Myocardium of L-NAME induced Hypertensive Rat

다국어 초록 (Multilingual Abstract)

The present study was designed to examine the antihypertensive effects of regular exercise by suppressing apoptosis in myocardium of L-NAME induced hypertension rats. We measured systolic and diastolic blood pressure (BP), and performed Bcl-2 Immunohistochemistry and TUNEL for apoptosis in heart tissue. Sprague-Dawley (n=40) rats were divided into 4 groups as follows: (1) control group (n=10, Con), (2) hypertension group(n=10, L-Con) (3) hypertension+exercise group (n=10, L-Ex), (4) Exercise group (n=10, Ex). To induce hypertension we administrated L-NAME (50 mg/kg) for 5days per week during 8weeks. The regular exercise was performed in swim water bath for 30min after 1hr L-NAME administration. Both L-NAME treatment and swim were performed 5 days /week with duration of 30minutes for 8weeks. BP was measured once per week. Results showed that the BP was significantly increased (p<.001) from 1week after L-NAME treatment, but systolic BP of L-Ex(p<.001) and diastolic BP of Ex(p<.05) groups showed markedly decreased from 4weeks and 2weeks after exercise, respectively. Particulary, the Bcl-2 expression in L-Ex was markedly increased as compared with L-Con group, whereas L-Con group showed decreased Bcl-2 expression and increased apoptotic cell than other groups. These results suggest that regular exercise would induce anti-hypertensive effect by controling the BP and preventing the apoptosis through bcl-2 expression in myocardium of L-NAME treated rats. Thus, regular exercise may be helpful to improvement of hypertensive heart function.

참고문헌 (Reference)

1 "novel molecular adaptation to systolic load in absence of hypertension" 423-9, 2000

2 "distinct models of cell death with fundamentally different significance" 229-259, 1982

3 "Toward an understanding of the molecular mechanism of physiological cell death" 786-789, 1993

4 "The role of exercise training in the treatment of hypertension" 2000

5 "The relationship of blood pressure to blood glucose and leisure time activity" 37-46, 1986

6 "The biochemistry of cell death by apoptosis" 1071-1074, 1990

7 "Sutural morphogenesis in the mouse calvaria the role of apoptosis" 74-80, 1985

8 "Stretch- mediated release of angiotensin II induces myocyte apoptosis by activating p53 that enhances the local renin -angiotensin system and decreases the Bcl-2 to Bax protein ratio in the cell" 1326-1342, 1998

9 "Stereology of the myocardium in hypertensive rats Differences in relation to the time of inhibition of nitric oxide synthesis" 369-373, 1998

10 "Sterelogy of cardiac hypertrophy induced by NO blockade in rats treated with enalapril and verapamil" 330-8, 2001

11 "Silent myocardial ischemia as apotential link between lack of premonitoring symptoms and increased risk of cardiac arrest during physical stress" 583-589, 1990

12 "Reperfusion injury induces apoptosis in rabbit cardiomyocytes" 1621-1628, 1994

13 "Regulation of fibrillar cillagen typer I and III and basement membrane type IV collagen gene expression in pressure overloaded rat myocardium" 787-794, 1990

14 "Prevention of apoptosis by bcl-2 release of cytochrome c from mitochondria blocked" 1129-1136, 1997

15 "Peptidase activities in rats treated chronically with N-nitro-L-arginine methyl ester" 205-214, 2004

16 "Patterns of physiological cell deaths and mitoses in the apical ectodermal ridge in normodactylous and polydactylous rat limb buds" 339-346, 1989

17 "Pathological versus physiological left ventricular hypertrophy a review" 129-141, 1998

18 "Oxidative injury due to chronic oxide synthase inhibition in rat effect of regular exercise on the heart" 75-82, 2002

19 "Nitric oxide, angiotensin II, and hypertension." 366-378, 2004

20 "Necrotic and apoptotic myocyte cell death in the aging heart of fischer 344 rats" 1996a

21 "Myocyte nuclear mitotic division and programmed myocyte cell death characterize the cardiac myopathy induced by rapid ventricular pacing in dogs" 771-787, 1995

22 "Medicine & Science in sports & Exercise" 533-553, 2004

23 "Low-intensity exercise training decreases cardiac output and hypertension in spontaneously hypertensive rats" -2631, 1997

24 "Interaction of physical training and chronic nitroglycerin treatment on blood pressure nitric oxide and oxidants/antioxidan -ts in the rat heart" 253-261, 2003

25 "Interaction between angiotensin II and nitric oxide during exercise in normal and heart failure rats" 7587-7599, 1999

26 "Induction of oxidative stress by glutathione Depletion Causes severe hypertension in normal rats" 142-146, 2000

27 "Increased vascular responsiveness to alpa 2-adrenergic stimula -tion during NOS inhibiton-induced hypertension" -2764, 1997

28 "Increased apoptosis in the heart of genetic hyperten sion associated with increased fibroblasts" 729-735, 2000

29 "Important role of tissue angiotensin-converting enzyme activity in the pathogenesis of coronary vascular and myocardial structural changes induced by long term blockade of nitric oxide synthesis in rats" 278-287, 1997

30 "High diastolic blood pressure during exercise id associated with hyper- cholesterolemia in patients with coronary artery disease" kubozo : 79-87, 2005

31 "Free radical, exercise, apoptosis, and heat shock proteins." 66-89, 2001

32 "Exercise training enhanced the expression of myocardial proteins related to cell protection in spontaneously hypertensive rats" 29-32, 2004

33 "Exercise causes a tissue-specific change of NO production in the kidney and lung" 60-68, 2003

34 "Effects of swim traning on blood pressure catecholamine and prostaglandin in spontaneously hyperten -sive rats" 1994

35 "Effects of exercise training on cardiac function and apoptosis in rats" gene expression -3002, 2000

36 "Effect of alacepril on blood pressure and neurohumoral factors at and during dynamic exercise in patiens with essential hypertension" ogi : 366-369, 1992

37 "Effect of AT1 receptor blockade on cardiac apoptosis in angiotensin II-induced hypertension" 2002

38 "Effect I midapril on Myocardial Remodeling in L-NAME -Induced Hypertensive Rats is associated With Gene Expression of NOS and ACE mRNA" 2000

39 "Development and regression of left ventricular hypertrophy" 1309-1320, 1984

40 "Control of skeletal muscle blood during dynamic exercise" 119-146, 1996

41 "Clinical Study on conducting physical exercise in lowering blood pressure of huypertensive patients and influencing their endocrine hormones" 897-899, 2001

42 "Chronic inhibition of nitric oxide synthesis causes coronary microvascular remodeling in rats" 957-62, 1995

43 "Chronic Nitric Oxid Inhibition Model six years on." Heart Association Inc 958-964, 1998

44 "Chondroclasts and endothelial cells collaborate in the process of cartilage resorption" 504-514, 1992

45 "Cardiac myocyte necrosis induced by angiotesin II" 1185-1195, 1991

46 "Blood pressure and hormone responses to aerobic exercise" 125-131, 1985

47 "Apoptotic and necrotic myocyte cell death are independent contributing variables of infarct size in rab invest" 86-107, 1996b

48 "Apoptosis, coronary arterial remodeling, and myocar-dial infarction after nitric oxide inhibition in SHR" 609-616, 1999

49 "Apoptosis in vasculature of spontaneously hypertensive rats Effect of an angiotensin converting enzyme inhibitoer and a calcium channel antagonist" 1108-1116, 1998

50 "Apoptosis in target organs of hypertension" 642-648, 1995

51 "Apoptosis in glomerular sclerosis" 103-111, 1996

52 "Angiotensin II Induces apoptosis of adult ventricular myocytes in vitro" 859-870, 1997

53 "A synchrondronous develop- ment of the rat colon" 573-578, 1991