상추잎 즙액과 표면에서 Escherichia coli O157:H7의 생장 가능성 = Growth Potential of Escherichia coli O157:H7 in Lettuce Leaf Extract and on Lettuce Leaf Surface

다국어 초록 (Multilingual Abstract)

This study was to evaluate the growth potential of E. coli O157:H7 in lettuce leaf extracts and on lettuce leaf surface at various temperatures. The pathogen can survive and multiply in the extracts and leaf surface of lettuce. The population of E. coli O157:H7 in the lettuce extracts reached to 4.79 log CFU/mL at 37℃. The multiplication of pathogen in lettuce extracts initiated within 10 hours of inoculation over 15℃ conditions. And it can survive in the lettuce leaf extracts at 4℃ for 100 hours at least. And this pathogen can multiply on lettuce leaf surface and the population of pathogen on the lettuce leaf surface increased to 1.82 log CFU/g at 25℃. At 37℃, the pathogen density increased to 1.53 CFU/g within 3 days after inoculation. At all temperature, irrespective of the inoculation level, similar trends in growth of E. coli O157:H7 were observed. These results emphasize the growth potential of E. coli O157:H7 in lettuce leaf extract and on lettuce leaf surface. To reduce the risk of outbreak, it is important to maintain the cold chain system during storage before the consumption.

참고문헌 (Reference)

1 식품의약품안전청, "식품의약품안전청 용역연구개발과제 최종보고서" 2009

2 "세계무역기구"

3 "미국 질병통제예방 센터"

4 통계청, "농작물생산조사"

5 Jennifer, L. K., "Transcriptome analysis of Escherichia coli O157:H7 exposed to lysates of lettuce leaves" 76 : 1375-1387, 2010

6 Lang M. M., "Survival and recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on lettuce and parsley as affected by method of inoculation, time between inoculation and analysis, and treatment with chlorinated water" 67 : 1092-1103, 2004

7 Abdul-Raouf, U. M., "Survival and growth of Escherichia coli O157:H7 on salad vegetables" 59 : 1999-2006, 1993

8 Marek, P, "Survival and growth characteristics of Escherichia coli O157:H7 in pasteurized and unpasteurized Cheddar cheese whey" 94 : 1-7, 2004

9 Brandl, M. T.:, "Plant lesions promote the rapid multiplication of Escherichia coli O157:H7 on postharvest lettuce" 74 : 5285-5289, 2008

10 Harapas, D., "Persistence of Escherichia coli on injured vegetable plants" 138 : 232-237, 2010

11 Choi, J. W., "Microbial contamination levels of fresh vegetables distributed in markets" 20 : 43-47, 2005

12 Brandl, M. T, "Leaf age as a risk factor in contamination of lettuce with Escherichia coli O157:H7 and Salmonella enterica" 74 : 2298-2306, 2008

13 Vinten, A. J. A, "Fate of Escherichia coli and Escherichia coli O157 in soil and drainage water following cattle slurry application at 3 sites in southern Scotland" 18 : 223-231, 2002

14 Janisiewicz, W. J., "Fate of Escherichia coli O157:H7 on fresh-cut apple tissue and its potential for transmission by fruit flies" 65 : 1-5, 1999

15 Jiang, X, "Fate of Escherichia coli O157:H7 in manure-amended soil" 68 : 2605-2609, 2002

16 Strawn, L. K, "Fate of Escherichia coli O157:H7 and Salmonella spp. on fresh and frozen cut mangoes and papayas" 138 : 78-84, 2010

17 Alegre, I., "Factors affecting growth of foodborne pathogens on minimally processed apples" 27 : 70-76, 2010

18 Mandrell, R. E, "Enteric human pathogens associated with fresh produce: sources, transport and ecology" IFT/Blackwell Publishing 3-42, 2009

19 Oliveira, M., "Effects of packaging type and storage temperature on the growth of foodborne pathogens on shredded ‘Romaine’ lettuce" 27 : 375-380, 2010

20 Samara, A, "Effect of treating lettuce surfaces with acidulants on the behavior of Listeria monocytogenes during storage at 5 and 20oC and subsequent exposure to simulated gastric fluid" 129 : 1-7, 2009

21 Wachtel, M. R, "Association of Escherichia coli O157:H7 with preharvest leaf lettuce upon exposure to contaminated irrigation water" 65 : 18-25, 2002