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다국어 초록 (Multilingual Abstract)

Copper and zinc are trace elements essential for the survival of organisms. Copper and zinc metal homeostasis was regulated with a specific mechanism. In this study, I investigated that the role of copper and zinc metal ion in other metal uptake system. I found that a growth defect caused by the deletion of afmac1, the copper response transcription factor, in Aspergillus fumigatus was recovered by zinc depletion. As a result, ZafA, the zinc response transcription factor, has a role in copper uptake. ZafA regulated the major copper transporter, CtrC transcription by binding to the motif of the promoter region (5'-CAA(G)GGT-3'). The CtrC transcription regulation of ZafA was independent of the Afmac1 regulating mechanisms to CtrC. The other copper transporter family genes RNA expressions were increased under zinc depletion conditions, but they’re not regulated by ZafA. Furthermore, Zinc and copper ions directly bind to ZafA. It's revealed that ZafA DNA binding activity is affected by not only zinc but also copper. The increasements of each metal ion were led ZafA to eliminate DNA binding. With continued research, I found that the afmac1 deletion has decreased RNA expression of the zinc transporters, zrfA and zrfC shows that impairments in the zinc uptake. Interestingly, zrfA, zrfC and zafA have the Afmac1 binding motif on the predicted region of the promoter. The zinc response genes, like zrfA, zrfC and zafA, transcriptions were downregulated in the afmac1 deletion under the zinc-deficient condition. Unexpectedly, I found that the zafA transcription level was increased in the afmac1 deletion under the copper-deficient condition. Collectively, I revealed that the two metals regulation metabolisms had raveled each other.

목차 (Table of Contents)

Contents
Contents і
List of figures ⅲ
General abstract 1
General introduction 3

Contents
Contents і
List of figures ⅲ
General abstract 1
General introduction 3
Reference 13
Chapter I 17
Abstract 18
Introduction 20
Material and method 25
Results 34
Discussion 70
Reference 74
Chapter II 81
Abstract 82
Introduction 83
Material and method 87
Results 93
Discussion 103
Reference 107
General conclusion 111
Abstract in Korean 113

참고문헌 (Reference)

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