Differential metabolism of juvenile hormone III between diapause and non-diapause of Aspongopus chinensis Dallas (Hemiptera: Dinidoridae) revealed by transcriptome sequencing

다국어 초록 (Multilingual Abstract)

Aspongopus chinensis Dallas, 1851 is an important insect resource with a long utilization history as traditional Chinese medicine and food owing to its various health benefits, including anti-cancer, anti-bacteria, and anticlotting properties. However, the long period of reproductive diapause during the overwintering stage has limited the broad utilization and artificial cultivation of A. chinensis. Diapause is largely regulated by juvenile hormones. Therefore, understanding the relationship between juvenile hormone metabolism and A. chinensis diapause may provide useful insight for developing genetic engineering strategies to regulate diapause. We identified differentially expressed genes in diapause and non-diapause adults of A. chinensis by transcriptome sequencing. A total of 336,230,260 clean reads were assembled into 80,769 unigenes. Overall, 3,524 differen tially expressed genes were identified, including 2,174 down-regulated and 1,350 up-regulated genes in diapause adults. Among these differentially expressed genes, 22 were significantly enriched in the JH III metabolismrelated pathway based on Kyoto Encyclopedia of Genes and Genomes analysis. These results provide insight into the molecular-level mechanism of diapause regulation and highlight new targets for preventing diapause to improve A. chinensis cultivation and productivity.

참고문헌 (Reference)

1 Deng, Y., "Transcriptome sequencing for identification of diapause-associated genes in fall webworm, Hyphantria cunea Drury" 668 : 229-236, 2018

2 Dong, Y. C., "Transcriptome characterization analysis of Bactrocera minax and new insights into its pupal diapause development with gene expression analysis" 10 : 1051-1063, 2014

3 T.S. Adams, "The rôle of juvenile hormone in housefly ovarian follicle morphogenesis" Elsevier BV 20 (20): 263-276, 1974

4 Yagi, S., "The Juvenile Hormones" Plenum Press 288-300, 1976

5 Jiang, S. J., "The Integration of Chinese Medicinal Insects" China Forestry Publishing House 131-, 1999

6 Zhang, L., "Study on the resources and utilization of Aspongonpus chinensis" 36 : 151-155, 2011

7 Chen, J., "Serpin7 controls egg diapause of migratory locust(Locusta migratoria)by regulating polyphenol oxidase" 10 : 707-717, 2020

8 Miyawaki, R., "Role of juvenile hormone in the control of summer diapause in adult Poecilocoris lewisi(Heteroptera : Scutelleridae)" 26 : 1-10, 2006

9 BG Unni, "Role of Insect Neuropeptides and Juvenile Hormones in Silk Protein Biosynthesis" SAGE Publications 15 (15): 112-117, 2008

10 David L. Denlinger, "Regulation of Diapause" Annual Reviews 47 (47): 93-122, 2002

11 Abrahim S. Caroci, "Reduced juvenile hormone synthesis in mosquitoes with low teneral reserves reduces ovarian previtellogenic development in Aedes aegypti" The Company of Biologists 207 (207): 2685-2690, 2004

12 Xiao-Xue Wang, "P–S6K is associated with insect diapause via the ROS/AKT/ S6K/CREB/HIF-1 pathway in the cotton bollworm, Helicoverpa armigera" Elsevier BV 120 : 103262-, 2020

13 Goto, S. G., "Photoperiodism: The Biological Calendar" Oxford University Press 258-286, 0011

14 Huang, X., "New players in the regulation of ecdysone biosynthesis" 35 : 1-10, 2008

15 Wei, C., "Morphological and biological characteristics of Aspongonpus chinensis" 34 : 26-30, 2015

16 Love, M. I., "Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2" 15 : 550-, 2014

17 Daniel A. Hahn, "Meeting the energetic demands of insect diapause: Nutrient storage and utilization" Elsevier BV 53 (53): 760-773, 2007

18 Parthasarathy, R., "Juvenile hormone regulation of vitellogenin synthesis in the red flour beetle, Tribolium castaneum" 40 : 405-414, 2010

19 Riddiford, L. M., "Juvenile hormone action : A 2007 perspective" 54 : 895-901, 2008

20 Shinoda, T., "Juvenile hormone acid methyltransferase : a key regulatory enzyme for insect metamorphosis" 100 : 11986-11991, 2003

21 Zachary A. Batz, "Juvenile Hormone III but Not 20-Hydroxyecdysone Regulates the Embryonic Diapause of Aedes albopictus" Frontiers Media SA 10 : 1352-, 2019

22 Vladimír Koštál, "Insect photoperiodic calendar and circadian clock: Independence, cooperation, or unity?" Elsevier BV 57 (57): 538-556, 2011

23 Bede, J. C., "Insect juvenile hormones in plants" 22 : 369-418, 2000

24 Denlinger, D. L., "Insect Endocrinology, 10" Academic Press 430-463, 2012

25 Harsimran Kaur Gill, "Insect Diapause: A Review" David Publishing Company 7 (7): 454-473, 2017

26 Cui, D. N., "Identification of diapause-associated proteins in migratory locust, Locusta migratoria L. (Orthoptera: Acridoidea) by label-free quantification analysis" 18 : 2579-2588, 2019

27 Cai, W. Z., "General Entomology" China Agriculture Press 306-307, 2011

28 Sahraeian, S. M. E., "Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis" 8 : 59-, 2017

29 Grabherr, M. G., "Full-length transcriptome assembly from RNA-Seq data without a reference genome" 29 : 644-652, 2011

30 Tao Jiang, "Expression analysis and functional identification of several genes related to diapause inBombyx mori" Wiley 61 (61): 150-157, 2019

31 BRIAN J. KOPPER, "Evidence for Reproductive Diapause in the Fritillary Speyeria idalia (Lepidoptera: Nymphalidae)" Oxford University Press (OUP) 94 (94): 427-432, 2001

32 Daniel A. Hahn, "Energetics of Insect Diapause" Annual Reviews 56 (56): 103-121, 2011

33 Robich, R. M., "Diapause in the mosquito Culex pipiens evokes a metabolic switch from blood feeding to sugar gluttony" 102 : 15912-15917, 2005

34 Ragland, G. J., "Developmental trajectories of gene expression reveal candidates for diapause termination : a key lifehistory transition in the apple maggot fly Rhagoletis pomonella" 214 : 3948-3960, 2011

35 Qi, X. Y., "De novo transcriptome sequencing and analysis of Coccinella septempunctata L. in nondiapause, diapause and diapause termination states to identify diapause-associated genes" 16 : 1086-, 2015

36 Chinzei, Y., "Cyanoprotein : Developmental stage, sex and diapause-dependent expression, and synthesis regulation by juvenile hormone in the bean bug, Riptortus clavatus" 20 : 61-73, 1992

37 Denlinger, D. L., "Comprehensive Molecular Insect Science" Elsevier 615-650, 2005

38 Hammock, B. D., "Comprehensive Insect Physiology, Biochemistry, and Pharmacology" Pergamon Press 431-472, 1985

39 Li, S. Z., "Compendium of Materia Medica" People’s Medical Publishing House, Beijing 1979

40 David S. Kang, "Comparative Transcriptomics Reveals Key Gene Expression Differences between Diapausing and Non-Diapausing Adults of Culex pipiens" Public Library of Science (PLoS) 11 (11): e0154892-, 2016

41 Chinese Pharmacopoeia Commission, "Chinese Pharmacopoeia" China Medical Science and Technology Press 11-, 2015

42 Li, S., "Advances in studies on chemical constituents, pharmacological effects and clinical application of Aspongopus chinensis" 45 : 303-311, 2020

43 Hodek, I., "Adult diapause in coleoptera" 2012 : 249081-, 2012

44 Lawrence I. Gilbert, "A Molecular Genetic Approach to the Biosynthesis of the Insect Steroid Molting Hormone" Elsevier 73 : 31-57, 2005