오이/흑종호박 접목에서 오이녹반모자이크바이러스의 비기주 대목인 흑종호박으로 이동 = Cucumber green mottle mosaic virus Moved into the Non-Host Figleaf Gourd Passing through Cucumber in Grafting System

다국어 초록 (Multilingual Abstract)

Cucumber green mottle mosaic virus (CGMMV) was not infected in figleaf gourd by sap inoculation. However
CGMMV was detected by RT-PCR from the figleaf gourd collected from a field growing cucumber grafted
onto figleaf gourd in Cheonan, Chungcheongnam Province in 2008. Which field showed 100% infection rate
of the virus disease. In the experiment grafted with cucumber onto figleaf gourd, transportation of CGMMV
through cucumber to figleaf gourd was confirmed by RT-PCR when the virus was mechanically inoculated on
the leaves of the cucumber. The amplified DNA concentration of the virus on electrophoresis gel was much
higher in the cucumber than in the figleaf gourd. However, the virus particles from the figleaf gourds were
not observed under electron microscopy, also sap of the figleaf gourds was not transmittable to Nicotiana
benthamiana. To identify the existence of CGMMV particle, the virus was purified from figleaf gourd and
cucumber growing together in the graft system. CGMMV solution extracted from the cucumber represented
a typical absorption spectrum of the virus but that from the figleaf gourd did not. Only a few CGMMV particles
were observed in the purified preparation from the figleaf gourd. These results confirmed that CGMMV
only passed through figleaf gourd in the grafting system. This study indicated that figleaf gourd is not a host
of CGMMV.

다국어 초록 (Multilingual Abstract)

Cucumber green mottle mosaic virus (CGMMV) was not infected in figleaf gourd by sap inoculation. However
CGMMV was detected by RT-PCR from the figleaf gourd collected from a field growing cucumber grafted
onto figleaf gourd in Cheonan, Chungcheongnam Province in 2008. Which field showed 100% infection rate
of the virus disease. In the experiment grafted with cucumber onto figleaf gourd, transportation of CGMMV
through cucumber to figleaf gourd was confirmed by RT-PCR when the virus was mechanically inoculated on
the leaves of the cucumber. The amplified DNA concentration of the virus on electrophoresis gel was much
higher in the cucumber than in the figleaf gourd. However, the virus particles from the figleaf gourds were
not observed under electron microscopy, also sap of the figleaf gourds was not transmittable to Nicotiana
benthamiana. To identify the existence of CGMMV particle, the virus was purified from figleaf gourd and
cucumber growing together in the graft system. CGMMV solution extracted from the cucumber represented
a typical absorption spectrum of the virus but that from the figleaf gourd did not. Only a few CGMMV particles
were observed in the purified preparation from the figleaf gourd. These results confirmed that CGMMV
only passed through figleaf gourd in the grafting system. This study indicated that figleaf gourd is not a host
of CGMMV.

참고문헌 (Reference)

1 고숙주, "오이 종자로부터 CGMMV의 검출과 품종 저항성 검정" 한국식물병리학회 10 (10): 154-158, 2004

2 최국선, "수박에 오이녹반모자이크바이러스의 토양전염과 예방대책" 한국식물병리학회 10 (10): 44-47, 2004

3 이상용, "물바이러스 실험서: 6-1-1 Tobacco mosaic virus 정제" 서운 124-124, 2000

4 Brunt,A.A., "Plant Viruses Online: Descriptions and Lists from the VIDE Database" he Australian National University 1996

5 Choi,G.S, "Occurrence of two tobamovirus diseases in cucurbits and control measures in Korea" 17 : 243-248, 2001

6 Lee,K.W., "Occurrence of cucumber green mottle mosaic virus disease of watermelon in Korea" 6 : 250-255, 1990

7 Ainworth,G.C, "Mosaic disease of cucumber" 22 : 55-67, 1935

8 Moreno,I.M., "Analysis of the systemic colonization of cucumber plants by Cucumber green mottle mosaic virus" 85 : 749-759, 2004