Phylogenetic Analysis of Mitochondrial DNA Control Region in the Swimming Crab, Portunus trituberculatus

다국어 초록 (Multilingual Abstract)

The control region of mitochondrial DNA (13516-
14619) is located between srRNA and tRNAlle gene in
swimming crab, Portunus trituberculatus. The present study
was investigated the genetic polymorphisms of the control
region in samples of P. trituberculatus collected at coastal
waters of the Yellow Sea in Korea. A total of 300 substitution
and indel polymorphic sites were identified. In addition to
SNPs and indel variation, a hypervariable microsatellite
motif was also identified at position from 14358 to 14391,
which exhibited 10 alleles including 53 different suballeles.
When the hypervariable microsatellite motif was removed
from the alignment, 95 haplotypes were identified (93 unique
haplotypes). The nucleotide and haplotype diversities were
ranged from 0.024 to 0.028 and from 0.952 to 1.000,
respectively. The statistically significant evidence for
geographical structure was not detected from the analyses
of neighbor-joining tree and minimum-spanning network,
neither. This result suggest that population of P. trituberculatus
are capable of extensive gene flow among populations. We
believed that the polymorphisms of the control region will be
used for informative markers to study phylogenetic relationships
of P. trituberculatus.

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