자연과학편 : 트레드밀 운동 중 경사도 조절에 의한 운동강도가 쥐의 세포사멸 관련 유전자에 미치는 영향 = Effects of exercise intensity determined by grade of treadmill on apoptosis-related gens in rats

국문 초록 (Abstract)

본 연구는 경사도 증가에 의한 운동강도에 따라 실험쥐의 골격근에 아몹토시스 관련 유전자인 caspase-3, bcl-2, bax, bcl-xl, bad, DNA fragmentation 변화를 알아보는데 목적이 있다 0%, 10%, 20% 경사도로 나누어 각각 50, 40, 30분 간 운동(속도고정; 2Qm/min)을 수행시켜 다음과 같은 결론을 얻었다. caspase-3, Bcl-2, Bad, Bax, bcl-xl mRNA, DNA fragmentation 은 다른 그룹과 비교하여 20% 경사도에서 가장 높았다. 20% 경사도 그룹내 변화에서 운동직후 에 가장 높게 증가한 것은 caspase-3, Bcl-2, bcl-xl이고, 회복기 5h에 가장 높게 증가한 것은 Bad, Bax, DNA fragmentation이다. 따라서 근수축 부하의 증가에 따라 아품토시스 관련 유전자의 변화는 차이가 있으며, 부하가 증 가할수록 아폽토시스에 의한 세포사멸이 나타난다.

다국어 초록 (Multilingual Abstract)

Exercise is associated with intensity-dependent muscle strengts and microinjury disturbances. Apoptosis may contribute to this phenomenon investigated the effects of different intensities determined by graded treadmill exercise on skeletal muscle of mice. The caspase-3, Bcl-2, Bax, Bcl-XL, Bad transcript contents were estimated by RT-PCR DNA laddering was used to detect DNA fragmentation as an indicator of apoptosis. The results of the study can summerized as follows ; caspase-3, Bcl-2, Bad, Bax, bcl-xl mRNA and DNA fragmentation was significantly peaked at 20% degree exercise group when compared with 0% and 10% degree exercise group. Also, the caspase-3, Bcl-2, bcl-xl at 20% degree exercise group was peaked that immediately when compared with rest, recovery 5h. Bad, Bax, DNA fragmentation at 20% degree exercise group was peaked that recovery 5h when compared with rest, immediately. It was concluded that load of muscle contraction increased that undergo cell death by apoptosis.

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