Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction

다국어 초록 (Multilingual Abstract)

Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

참고문헌 (Reference)

1 Vaughan, C., "The Jarisch-Herxheimer reaction in leptospirosis" 70 : 118-121, 1994

2 Wagenaar, J. F., "Soluble ST2 levels are associated with bleeding in patients with severe leptospirosis" 3 (3): e453-, 2009

3 Murray, G. L., "Mutations affecting Leptospira interrogans lipopolysaccharide attenuate virulence" 78 : 701-709, 2010

4 Isogai, E., "Macrophage activation by leptospiral lipopolysaccharide" 273 : 200-208, 1990

5 De Haas, C. J. C., "Lipopolysaccharide (LPS)-binding synthetic peptides derived from serum amyloid P component neutralize LPS" 67 : 2790-2796, 1999

6 Kemenes, F., "Leptospirosis of the swine" 22 : 224-233, 1967

7 Guo, Y., "Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts" 2014

8 Werts, C., "Leptospiral lipopolysaccharide activates cells through a TLR2-dependent mechanism" 2 : 346-352, 2001

9 Faine, S., "Leptospira and leptospirosis" CRC Press 1994

10 De Fost, M., "Interleukin 12 in part regulates gamma interferon release inhuman whole blood stimulated with Leptospira interrogans" 10 : 332-335, 2003

11 Rathi, A. V., "Induction of interferon-stimulated genes by simian virus 40 T antigens" 406 : 202-211, 2010

12 Yan, W., "Immunogenicity andprotective efficacy of recombinant Leptospira immunoglobulin- like protein B (rLigB) in a hamster challenge model" 11 : 230-237, 2009

13 Brenner, D. J., "Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies" 49 : 839-858, 1999

14 Lowanitchapat, A., "Expression of TNF-alpha, TGF-beta, IP-10 and IL-10 mRNA in kidneys of hamsters infected with pathogenic Leptospira" 33 : 423-434, 2010

15 Uddin, M. J., "Expression dynamics of Toll-like receptors mRNA and cytokines in porcine peripheral blood mononuclear cells stimulated by bacterial lipopolysaccharide" 147 : 211-222, 2012

16 Priya, C. G., "Evidence for endotoxin as a causative factor for leptospiral uveitis in humans" 49 : 5419-5424, 2008

17 Fukuda, T., "Efficient establishment of pig embryonic fibroblast cell lines with conditional expression of the simian vacuolating virus 40 large T fragment" 76 : 1372-1377, 2012

18 Isogai, E., "Effects of leptospiral lipopolysaccharide on rabbit platelets" 271 : 186-196, 1989

19 Pollack, M., "Dual effects of LPS antibodies on cellular uptake of LPS and LPS-induced proinflammatory functions" 159 : 3519-3530, 1997

20 Isogai, E., "Biological activities of leptospiral lipopolysaccharide" 261 : 53-64, 1986

21 Isogai, E., "Apoptosis of lymphocytes in mice dministered lipopolysaccharide from Leptospira interrogans" 45 : 529-537, 1998

22 Fukuda, T., "A new western blotting method using polymer immunocomplexes: detection of Tsc1 and Tsc2 expression in various cultured cell lines" 285 : 274-276, 2000

23 Cerqueira, G. M., "A century of Leptospira strain typing" 9 : 760-768, 2009