秘傳順氣散이 腦血流와 一過性 腦損傷에 미치는 影響 = (The) Effect of Bijeonsungi-San on Regional Cerebral Blood Flow and Transient Brain Injury in Rats

다국어 초록 (Multilingual Abstract)

This Study was designed to investigate the effect of Bijeonsungi-San freeze dry powder(BSS) on the change of cerebral hemodynamics〔regional cerebral blood flow(rCBF) and mean arterial blood pressure(MABP)〕in normal and cerebral ischemic rats, and further to determine the mechanisms of action of BSS on hemodynamics. In addition, this study was designed to investigate whether BSS inhibits lactate dehydrogenase(LDH) activity in neuronal cells and cytokines production in serum of cerebral ischemic rats.
The results were as follows ;
1. BSS significantly increased rCBF in a dose-dependent manner, but did not change MABP. These results suggest that BSS significantly increases rCBF by dilating pial arterial diameter.
2. The BSS-induced increase in rCBF was significantly inhibited by pretreatment with methylene blue(MTB, 10 ㎍/㎏, i.p.), an inhibitor of guanylate cyclase and inhibited by pretreatment with indomethacin(IDN, 1 ㎎/㎏, i.p.), an inhibitor of cyclooxygenase. These results suggest that the mechanism of action of BSS is mediated by guanylate cyclase.
3. The BSS-induced change in MABP was not affected by pretreatment with MTB and IND.
4. The change of rCBF was significantly and stably increased by BSS(10 ㎎/㎏, i.p.) during the period of cerebral reperfusion, which contrasted with the findings of rapid and marked increase in control group.
5. BSS significantly inhibited LDH activity in neuronal cells. These results suggest that BSS prevents the neuronal death.
6. In cytokine production in the serum drawn from femoral artery after 1 hr of middle cerebral arterial occlusion, sample group showed significantly decreased production of interleukin(IL)-1β production, decreased production of tumor necrosis factor(TNF)-α and significantly increased production of IL-10 and transforming growth factor(TGF)-β compared with control group.
7. In cytokine production in the serum drawn from femoral artery after 1 hr of reperfusion, sample group showed significantly decreased production of IL-1β, decreased production of TNF-α and significantly increased production of IL-10 and TGF-β compared with control group.
These results suggest that BSS mediated by guanylate cyclase has inhibitive effect on the brain damage by inhibiting LDH activity, IL-1β and TNF-α production and by accelerating IL-10 and TGF-β production.
The present author thinks that BSS has an anti-ischemic effects through the improvement of cerebral hemodynamics and inhibitive effects on the brain damage.

목차 (Table of Contents)

• 目次
• ABSTRACT = ⅰ
• Ⅰ. 緖論 = 1
• Ⅱ. 材料 및 方法 = 2
• 1. 재료 = 2
• 1) 동물 = 2
• 2) 약재 = 2
• 2. 방법 = 4
• 1) 검액의 제조 = 4
• 2) 정상 흰쥐의 뇌혈류역학 변화 관찰 = 4
• 3) 정상 흰쥐의 뇌혈류역학 변화에 미치는 기전 관찰 = 5
• 4) 뇌허혈 흰쥐의 뇌혈류역학 변동에 미치는 개선 효과 관찰 = 5
• 5) 생쥐의 신경세포내 LDH 활성도 측정 = 6
• 6) 뇌허혈 흰쥐의 혈청내 사이토카인 생성능 측정 = 7
• 3. 통계분석 = 9
• Ⅲ. 實驗成績 = 10
• 1. 秘傳順氣散이 정상 흰쥐의 뇌혈류역학에 미치는 효과 = 10
• 2. 秘傳順氣散 투여로 변화된 정상 흰쥐의 뇌혈류역학 작용기전 관찰 = 12
• 1) 秘傳順氣散 투여로 변화된 정상 흰쥐의 국소 뇌혈류량 작용기전 관찰 = 12
• 2) 秘傳順氣散 투여로 변화된 정상 흰쥐의 평균혈압 작용기전 관찰 = 14
• 3. 秘傳順氣散이 뇌허혈 흰쥐의 국소 뇌혈류량 변동에 미치는 개선 효과 = 16
• 4. 秘傳順氣散이 생쥐의 신경세포의 LDH 활성도에 미치는 효과 = 18
• 5. 秘傳順氣散이 뇌허혈 흰쥐의 혈청에서 생성되는 사이토카인 양에 미치는 효과 = 20
• 1) 중대뇌동맥 폐색 1 시간 후 생성되는 사이토카인 양에 미치는 효과 = 20
• 2) 재관류 1 시간 후 생성되는 사이토카인 양에 미치는 효과 = 22
• Ⅳ. 考察 = 24
• Ⅴ. 結論 = 30
• 參考文獻 = 32