Reponses of the Hepatic Microsomal Cytochrome P450 Monooxygenase System in Rock Bream Oplegnathus fasciatus Exposed to Tributyltin (TBT)

다국어 초록 (Multilingual Abstract)

The study was conducted to investigate the responses of the hepatic microsomal cytochrome P450 monooxygenase system in the rock bream Oplegnathus fasciatus after chronic exposure to 0, 1, 2, 4, and 8 μg/L tributyltin (TBT) concentrations for 4 weeks.
Hepatic cytochrome 450 content and ethoxyresorufin O-deethylation (EROD) activity were found to significantly increase in fish treated with the higher concentration of TBT (≥4 μg/L); however, no significant changes were observed in penthoxyresorufin Odeethylation (PROD) activity in all treated groups compared to the control group. These findings suggest that exposure to a low TBT concentration (≥4 μg/L) has the potential to induce cytochrome 450 content and EROD enzyme activity in hepatic tissue inthe rock bream.

참고문헌 (Reference)

1 Goksøyr A, "Use of cytochrome P450 1A (CYP1A) in fish as a biomarker of aquatic pollution" 17 : 80-95, 1995

2 Fent K, "Tributyltin-induced effects on early life stages of minnows, Phoxinus phoxinus" 22 : 429-438, 1992

3 Ric CD, "Tributyltin modulates 3,3’,4,4’,5-pentachlorobiphenyl (PCB-126)-induced hepatic CYP1A activity in channel catfish, Ictalurus punctatus" 55 : 197-212, 1998

4 Wang DY, "Toxic effects of tributyltin (TBT) on early life stage of thornfish (Terapon jarbua Forsskål)" 25 : 15-25, 1998

5 Wrighton SA, "The human hepatic cytochrome P-450 involved in drug metabolism" 22 : 1-22, 1992

6 Goksøyr A, "The cytochrome P450 system in fish, aquatic toxicology and environmental monitoring" 22 : 287-312, 1992

7 Omura T, "The carbon monoxide-binding pigment of liver microsomes. 2. Solubilization, purification and properties" 239 : 2379-2385, 1964

8 Stegeman JJ, "Structure, function and regulation of cytochrome P450 forms in fish" 18 : 19-21, 1990

9 Kennedy SW, "Simultaneous measurement of cytochrome P4501A catalytic activity and total protein concentration with a fluorescence plate" 222 : 217-223,

10 Gibbs PE, "Sex change in the female dog-whelk, Nucella lapillus around southwest England: Evidence for the effect of tributyltin from antifouling paints" 68 : 715-731, 1988

11 Skaare JU, "Response of xenobiotic metabolizing enzymes of rainbow trout (Oncorhynchus mykiss) to the mono-ortho substituted polychlorinated PCB congener 2, 3’, 4, 4’, 5-pentachlorobiphenyl, PCB-118, detected by enzyme activities and immunochemical methods" 20 : 349-352, 1991

12 Diez S, "Organotin contamination in sediments from the Western Mediterranean enclosures following 10 years of TBT regulation" 36 : 905-918, 2002

13 Horiguchi T, "Organotin compounds and their effects on aquatic organisms, focusing on imposex in gastropods" 17 (17): 81-100, 1994

14 Lu AYH, "Multiplicity of mammalian microsomal cytochrome P-450" 31 : 277-295, 1980

15 Brown JF, "Metabolic alteration of PCB residues in aquatic fauna: distributions of cytochrome P4501A1- and P4502B-like activities" 34 : 261-266, 1992

16 Yolanda M, "Interaction of tributylyin with hepatic cytovhrome P450 and uridine diphosphate-glucuronosyl tranfetase system of fish" 23 (23): 990-996, 2004

17 Morcillo Y, "Interaction of tributyltin- and triphenyltin with the microsomal monooxygenase system of mollusks and fish from the NW Mediterranean" 38 : 35-46, 1997

18 Fent K, "Inhibition of hepatic microsomal monooxygenase system by organotins in vitro in freshwater fish" 28 : 107-126, 1994

19 Oehlmann J, "Imposex in Nucella lapillus and intersex in Littorina littorea: interspecific comparison of two TBT-induced effects and their geographical uniformity" 378 : 199-213, 1998

20 Whyte JJ, "Ethoxyresorufin-Odeethylase (EROD) activity in fish as a biomarker of chemical exposure" 30 : 347-570, 2000

21 Xie J, "Effects of anthraquinone extract from rhubarb Rheum officinal Bail on the crowding stress response and growth of common carp Cyprinus carpio var. Jian" 281 : 5-11, 2008

22 Byrne P, "Effects of a cationic detergent on the gills and blood chemistry of rainbow trout Salmo gairdneri" 6 : 195-196, 1989

23 Brack W, "Effect-directed fractionation and identification of cytochrome P450A-inducing halogenated aromatic hydrocharbons in a contaminated sediment" 21 : 2654-2662, 2002

24 Mc Allister B, "Early life exposure to environmental levels of the aromatase inhibitor tributyltin causes masculinisation and irreversible sperm damage in zebrafish (Danio rerio)" 65 : 309-316, 2003

25 Haasch ML, "Cloned rainbow trout liver P450 complementary DNA as a potential environmental monitor" 98 : 362-368, 1989

26 Stegeman JJ, "Biochemistry and molecular biology of monooxygenases: Current perspectives on forms, functions, and regulation of cytochrome P450 in aquatic species, In Aquatic Toxicology Molecular Biochemical and Cellular Perspectives" Lewis 87-206, 1994

27 Livingstone DR, "Aquatic environmental biomonitoring: Use of cytochrome P450 1A and other molecular biomarkers in fish and mussels, In Environmental Biomonitoring: The Biotechnology Ecotoxicology Interface Vol 6-Biotechnology Reserch Series" Cambridge University Press 101-129, 1998

28 Tsuda T, "Accumulation and excretion of tri-n-butyltin chloride and tributyltin chloride by willow shiner" 101C : 67-70, 1992

29 Bradford M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" 72 : 248-245, 1976

30 Schwaiger J, "A prolonged toxicity study on the effects of sublethal concentration of bis (tri-n-butyltin) oxide (TBTO): histopathological and histochemical findings in rainbow trout (Oncorhynchus mykiss)" 23 : 31-48, 1992

31 Collier TK, "A field evaluation of cytochrome P4501A as a biomarker of contaminant exposure in three species of flat fish" 14 : 143-152, 1995