토양에서 분리한 Aspergillus sp. PS-104 균주에 의한 난용성 인산염 분해 = Original Research Article : Soil Environment ; Solubilization of Insoluble Phosphates by Aspergillus sp. PS-104 Isolated from Soil

다국어 초록 (Multilingual Abstract)

Phosphate-solubilizing microorganisms were isolated from soil around Kyungnam and Kyungbook regions using potato dextrose agar-calcium phosphate medium. A fungus with the greatest phosphate-solubilizing activity was selected and identified to Aspergillus sp. PS-104, based on the morphological characteristics of conidiophore and conidia; unbranching type of conidiophore, terminally swelling of conidiophore and septate of mycelium, in malt extract agar and potato dextrose agar media. The optimum temperature and initial pH to solubilize rock phosphate in potato dextrose broth-rock phosphate medium were 30℃ and pH 7.0, respectively. In these optimum conditions, phosphate-solubilizing activities of Aspergillus sp. PS-104 against four types of insoluble phosphate, tricalcium phosphate, aluminium phosphate, hydroxyapatite and rock phosphate, were quantitatively determined. As results, the maximum phosphate-solubilizing activity was obtained with tricalcium-phosphate (1,900 ppm) while minimum activity was obtained with hydroxyapatite (320 ppm). Futhermore, phosphate-solubilizing activity of Aspergillus sp. PS-104 was found higher when treated with nitrates as compared to the ammonium salts as a nitrogen sources.

참고문헌 (Reference)

1 "as inoculant and their role in augmenting crop productivity in India - A review. Crop Res. Hisar. 5" 11-, 1992

2 "Solubilization of natural rock phosphates and pure insoluble inorganic phosphates by Aspergillus awamori. Ind. J. Exp. Biol. 31" 747-749, 1993

3 "Solubilization of insoluble phosphates by Pseudomonas putida, Penicillium sp. and Aspergillus niger isolated from Korean soils" 28 : 278-286, 1995

4 "Solubilization of insoluble phosphates by Penicillium sp. GL-101 isolated from soil" 40 : 329-333, 1997

5 "Solubilization of insoluble metal compounds by soil fungi: Development of a screening method for solubilizing ability and metal tolerance" 99 : 987-993, 1995

6 "Solubilization of insoluble inorganic phosphate by a soil-inhabiting fungus Fomitopsis sp. PS 102" 82 : 439-442, 2002

7 "Solubilization of inorganic calcium phosphates-solubilization mechanisms" 27 : 257-263, 1995

8 "Solubilization of hardly-soluble AlPO4 with P-solubilizing microorganisms" 27 : 265-270, 1995

9 "Solid culture of phosphate-solubilizing fungus, Penicillium sp. PS-113" 27 : 1-7, 1999

10 "Rock phosphate-superphosphate in wheat in relation to inoculation with phosphate solubilizing organism and organic waste. Ind. J. Agr. Res. 27" 137-145, 1993

11 "Response of groundnut to phosphate solubilizing microorganisms. Groundnut News 6" 5-, 1994

12 "Mineral phosphate solubilization by Aspergillus aculeatus" 33 : 91-93, 1995

13 "Effect of Penicillium bilaji on the solubility and uptake of P and micronutrients from soil by wheat. Can. J. Soil Sci. 68" 261-270, 1988

14 "Ainsworth & Bisby's Dictionary of the Fungi" 1983