Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet = Effect of Lactobacillus rhamnosus hsryfm 1301 on the Gut Microbiota and Lipid Metabolism in Rats Fed a High-Fat Diet

다국어 초록 (Multilingual Abstract)

Accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. To investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed using real-time PCR before and after administration of Lactobacillus rhamnosus hsryfm 1301 and its fermented milk for 28 days. The findings showed that the Lactobacillus spp., Bifidobacterium spp., Bacteroides spp., and Enterococcus spp. content in the hyperlipidemic rats gut was increased significantly (p < 0.05), while the Clostridium leptum and Enterobacter spp. content was decreased significantly after intervening with L. rhamnosus hrsyfm 1301 and its fermented milk for 28 days (p < 0.05). Furthermore, the lipid levels of the serum and the liver were decreased significantly (p < 0.05) and the fecal water content was increased significantly (p < 0.05) in the hyperlipidemic rats after the intervention, and hepatocyte fatty degeneration of liver tissues was also prevented. A positive correlation was observed between the Clostridium leptum content and the level of serum cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, and a negative correlation was observed between the Enterobacter spp. content and the Lactobacillus spp. and Bifidobacterium spp. content in the hyperlipidemic rats gut. These results suggest that the gut microbiota and lipid metabolism of hyperlipidemic rats could be improved by supplementation with L. rhamnosus hsryfm 1301 and its fermented milk.

참고문헌 (Reference)

1 Matsuki T, "Use of 16S rRNA gene-targeted group-specific primers for real-time PCR analysis of predominant bacteria in human feces" 70 : 7220-7228, 2004

2 Kim M, "The water-soluble extract of chicory influences serum and liver lipid concentrations, cecal shortchain fatty acid concentrations and fecal lipid excretion in rats" 128 : 1731-1736, 1998

3 Tappenden KA, "The physiological relevance of the intestinal microbiota-contributions to human health" 26 : 679S-683S, 2007

4 Pryde SE, "The microbiology of butyrate formation in the human colon" 217 : 133-139, 2002

5 Ohtani O., "The maceration technique in scanning electron microscopy of collagen fiber frameworks: its application in the study of human livers" 55 : 225-225, 1992

6 Begley M, "The interaction between bacteria and bile" 29 : 625-651, 2005

7 McNulty NP, "The impact of a consortium of fermented milk strains on the gut microbiome of gnotobiotic mice and monozygotic twins" 3 : 106-, 2011

8 Armstrong MJ, "The hydrophobic-hydrophilic balance of bile salts. Inverse correlation between reversephase high performance liquid chromatographic mobilities and micellar cholesterol-solubilizing capacities" 23 : 70-80, 1982

9 Fava F, "The gut microbiota and lipid metabolism: implications for human health and coronary heart disease" 13 : 3005-3021, 2006

10 Pridmore RD, "The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii N C C 533" 101 : 2512-2517, 2004

11 Juang I, "The effect of oligosaccharide beverage on microflora in rats" 25 : 232-242, 2000

12 Turnbaugh PJ, "The effect of diet on the human gut microbiome: a metagenomic analysis in humanized gnotobiotic mice" 1 : 6ra14-, 2009

13 Tannock GW, "Studies of the intestinal microflora: a prerequisite for the development of probiotics" 8 : 527-533, 1998

14 Cani PD, "Selective increases of bifidobacteria in gut microflora improve high-fat-diet-induced diabetes in mice through a mechanism associated with endotoxaemia" 50 : 2374-2383, 2007

15 Kajiura K, "Relationship between fecal bile acids and the occurrence of colorectal neoplasia in experimental murine ulcerative colitis" 59 : 69-72, 1998

16 Fernandez M, "Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract" 94 : 449-455, 2003

17 Jones SE, "Probiotic Lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors" 9 : 35-, 2009

18 Hayashi H, "Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture - based methods" 46 : 535-548, 2002

19 Fite A, "Identification and quantitation of mucosal and faecal desulfovibrios using real time polymerase chain reaction" 53 : 523-529, 2004

20 Kim K-A, "High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway" 7 : e47713-, 2012

21 Gómez-Hurtado I, "Gut microbiota dysbiosis is associated with inflammation and bacterial translocation in mice with CCl4-induced fibrosis" 6 : e23037-, 2011

22 Ben-Amor K, "Genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16S rRNA gene analysis" 71 : 4679-4689, 2005

23 Yin Y-N, "Effects of four Bifidobacteria on obesity in high-fat diet induced rats" 16 : 3394-, 2010

24 Audisio MC, "Effect of different complex carbon sources on growth and bacteriocin synthesis of Enterococcus faecium" 63 : 235-241, 2001

25 Macfarlane G, "Effect of different carbohydrates on growth, polysaccharidase and glycosidase production by Bacteroides ovatus, in batch and continuous culture" 68 : 179-187, 1990

26 Núñez IN, "Effect of a probiotic fermented milk on the thymus in Balb/c mice under nonsevere protein–energy malnutrition" 110 : 500-508, 2013

27 Eckburg PB, "Diversity of the human intestinal microbial flora" 308 : 1635-1638, 2005

28 Larkin TA, "Dietary combination of soy with a probiotic or prebiotic food significantly reduces total and LDL cholesterol in mildly hypercholesterolaemic subjects" 63 : 238-245, 2007

29 Jones ML, "Cholesterol-lowering efficacy of a microencapsulated bile salt hydrolase-active Lactobacillus reuteri NCIMB 30242 yoghurt formulation in hypercholesterolaemic adults" 107 : 1505-1513, 2012

30 Bartosch S, "Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota" 70 : 3575-3581, 2004

31 WHO, "Cardiovascular Disease. In Fact sheet N°317"

32 An HM, "Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats" 10 : 116-, 2011

33 Malinen E, "Analysis of the fecal microbiota of irritable bowel syndrome patients and healthy controls with real-time PCR" 100 : 373-382, 2005

34 Turnbaugh PJ, "An obesity-associated gut microbiome with increased capacity for energy harvest" 444 : 1027-1131, 2006

35 Meki A-RM, "Aflatoxin B1 induces apoptosis in rat liver: protective effect of melatonin" 22 : 417-426, 2001

36 Bry L, "A model of host-microbial interactions in an open mammalian ecosystem" 273 : 1380-1383, 1996

37 Qin J, "A human gut microbial gene catalogue established by metagenomic sequencing" 464 : 59-65, 2010

38 Xu J, "A genomic view of the human-Bacteroides thetaiotaomicron symbiosis" 299 : 2074-2076, 2003