Two linear DNA killer plasmids from Kluyveromyces lactis were transfered into four kinds of secretion mutant derived from Saccharomyces cerevisiae(a,suc2, mal, gal2, cup1, rho) by cell fusions.
Out of 286 colonies regenerated on selective medium, tw...
Two linear DNA killer plasmids from Kluyveromyces lactis were transfered into four kinds of secretion mutant derived from Saccharomyces cerevisiae(a,suc2, mal, gal2, cup1, rho) by cell fusions.
Out of 286 colonies regenerated on selective medium, two strains derived from sec 7 and sec 1, three strains from sec 18, and two strains from sec 53 were identified harboring two killer plasmids by killer test and electro phoretic analysis of plasmids.