미세먼지 PM10이 피부각질형성세포와 피부섬유아세포에 미치는 세포생존율 분석 = The Survival Rate of Cultured Epithelial Keratinocyte Cell and Dermal Fibroblast Cell treated with Particulate matter(PM10)

다국어 초록 (Multilingual Abstract)

Particulate matter (PM) is a mixture of particles suspended in the polluted air. Recently it was revealed that PM influences upon the biological environment of the skin. It was not well-known about the mechanisms and changes from the keratinocytes exposed with PM.
We analyzed the effects of PM on cultured human keratinocyte cell (HaCaT) and human skin fibroblast cell (HS68). PM 10 was exposed to cultured HaCaT cell and CCD.-986Sk cell in various concentrations (10, 50, 100, 250, 500 μg/mL) for 24 and 48 hrs. MTT assay was done for measuring cytotoxicity on HaCaT and HS68 cells. The 24 and 48h exposure of HaCaT cells to increasing PM concentrations (10, 50, 100, 250, 500 μg/mL) changed cell viability. MTT-assay revealed a dose-dependent relation between the level of PM and cellular viability, with a statistically significant decrease after exposure of HaCaT cells. HaCaT cells were more viable compared to HS68 cells incubated with the same conditions. In conclusion, PM10 had a harmful effect on HaCaT and HS68 cells in terms of cytotoxicity.

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