붉은대게 폐각으로부터 키토산의 제조 및 물리화학적 특성 = Preparation and Physicochemical Properties of Chitosan from Red Crab Waste-Shell

다국어 초록 (Multilingual Abstract)

Chitosans were prepared from red crab chitin under various alkali treatment conditions(different alkali concentrations, reaction times and temperatures) and theirphysicochemical properties were investigated. The nitrogen content and deacetylation degree of red crab chitin were 6.15% and 22.17A%, respectively. By the IR spectra, red crab and reference chitin showed the sharp bands at 1650 CM-1/ and 1550 CM-1/, which are characteristic of chitin. The nitrogen contents of prepared chitosans ranged from 6.19~7.48%. Thedeacetylation degree was increased from 63~76% and 48~78% with increasing reaction time and temperature, whereas viscosity was decreased. The nitrogen content and yield of red crab chitosan perpared from chitin with 50% NaOH, 1:25(w/v) for 3.0 hr at 120CM-1 / were 7.26% and 85.0%, respectively. and viscosity, deacetylation degree and molecular weight, 67.0 mPa.s, 75.0% and 6.5x105 Dalton, respectively. From the IR spectra, the amide absorption bands of red crab and reference chitosan became very weak, similarly. And at solid state 13C-NMR spectra, C=O(carbonyl carbon) signals absent, whereas CH3(methyl carbon) was residues. Chemical shift of 13C-NMR spectra of red crab and reference chitosans were in good agreement with slight experimental deviation.