Two Rhizobacterial Strains, Individually and in Interactions with Rhizobium sp., Enhance Fusarial Wilt Control, Growth, and Yield in Pigeon Pea

다국어 초록 (Multilingual Abstract)

A Pseudomonas aeruginosa strain, RRLJ 04, and a Bacilluscereus strain, BS 03, were tested both individually and in combinationwith a Rhizobium strain, RH 2, for their ability toenhance plant growth and nodulation in pigeon pea (Cajanuscajan L.) under gnotobiotic, greenhouse and field conditions.
Both of the rhizobacterial strains exhibited a positive effecton growth in terms of shoot height, root length, fresh and dryweight, nodulation and yield over the non-treated control.
Co-inoculation of seeds with these strains and RhizobiumRH 2 also reduced the number of wilted plants, when grownin soil infested with Fusarium udum. Gnotobiotic studies confirmedthat the suppression of wilt disease was due to thepresence of the respective PGPR strains. Seed bacterizationwith drug-marked mutants of RRLJ 04 and BS 03 confirmedtheir ability to colonize and multiply along the roots. Theresults suggest that co-inoculation of these strains with Rhizobiumstrain RH 2 can be further exploited for enhancedgrowth, nodulation and yield in addition to control of fusarialwilt in pigeon pea.

참고문헌 (Reference)

1 "Vision 2030" Indian Institute of Pulses Research 42-, 2011

2 Ghosh, S., "Three newly isolated plant growth-promoting bacilli facilitate the seedling growth of canola, Brassica campestris" 41 : 277-281, 2003

3 Dutta, S., "Stress Biology" Narosa Publishing House 247-251, 2005

4 Parmar, N., "Stimulation of nitrogen fixation and induction of flavonoid like compounds by rhizobacteria" 86 : 36-44, 1999

5 Podile, A. R, "Seed bacterization with Bacillus subtilis AF 1 enhances seedling emergence, growth and nodulation in pigeon pea" 35 : 199-204, 1995

6 Rani, M.U., "Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea" 11 : 8085-8091, 2012

7 Kutcher, H. R., "Rhizobium inoculant and seed-applied fungicide effects on field pea production" 82 : 645-651, 2002

8 Perez, J. C., "Returning to Our Roots" 3-9, 2012

9 Sarig, S., "Promoting of leaf area development and yield in Sorghum bicolor inoculated with Azospirillum brasilense" 9 : 235-245, 1990

10 Vidhyasekaran, P., "Powder formulations of Pseudomonas fluorescens to control pigeon pea wilt" 8 : 166-171, 1997

11 Dileep Kumar, B. S., "Potential for improving pea production by co-inoculation with fluorescent Pseudomonas and Rhizobium" 225 : 25-34, 2001

12 Burd, G. I., "Plant growth-promoting bacteria that decrease heavy metal toxicity in plants" 46 : 237-245, 2000

13 Dashti, N., "Plant growth promoting rhizobacteria accelerate nodulation and increase nitrogen fixation activity by field grown soybean (Glycine max (L.) Merr.) under short season conditions" 200 : 205-213, 1998

14 Ogoshi, A., "Plant Growth–Promoting Rhizobacteria – Present Status and Future Prospects" 483-, 1997

15 Reddy, M. V., "Pigeon pea: Disease management, Pigeonpea" C A B Int. 303-307, 1990

16 Penrose, D.M., "Levels of 1-aminocyclopropane-1-carboxylic acid (ACC) in exudates and extracts of canola seeds treated with plant growth-promoting bacteria" 47 : 368-372, 2001

17 Geetha, R., "Isolation and characterization of nodule-associated Exiguobacterium sp. from the root nodules of Fenugreek (Trigonella foenum-graecum) and their possible role in plant growth promotion" 3 : 1-8, 2012

18 Estevez De Jensen, C., "Integrated management strategies of bean root rot with Bacillus subtilis and Rhizobium in Minnesota" 74 : 107-115, 2002

19 Dutta, S., "Induction of systemic resistance against fusarial wilts in pigeon pea through interaction of plant growth promoting rhizobacteria and rhizobia" 40 : 452-461, 2008

20 Saini, P., "Evaluation of native rhizobacteria as promoters of plant growth for increased yield in lentil (Lens culinaris)" 4 : 05-09, 2012

21 Nukui, N., "Effects of ethylene precursor and inhibitors for ethylene biosynthesis and perception on nodulation in Lotus japonicus and Macroptilium atropurpureum" 41 : 893-897, 2000

22 Wang, C., "Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes into Pseudomonas fluorescens strain CHA0 and its gacA derivative CHA96 on their growth-promoting and disease-suppressive capacities" 46 : 898-907, 2000

23 Roopa, B., "Effect of different PGPR strains along with Rhizobium on nodulation and chickpea productivity" 3 : 424-426, 2012

24 Leij, F. A. A. M. De., "Effect of 2,4-diacetylphloroglucinol-producing and non-producing strains of Pseudomonas fluorescens on root development of pea seedlings in three different soil types and its effect on nodulation by Rhizobium" 35 : 114-121, 2002

25 Dileep Kumar, B. S, "Disease suppression and crop improvement through fluorescent pesudomonads isolated from cultivated soils" 14 : 735-741, 1998

26 Raj, S. N., "Comparative performance of formulations of PGPR in growth promotion and suppression of downy mildew in pearl millet" 22 : 579-588, 2003

27 Singh, R., "Coinoculation of Rhizobium and Pseudomonas strains isolated from Trigonella foenum graecum (Methi) and check their PGPR potential" 2012

28 Siddiqui, I. A., "Co-inoculation of Pseudomonas aeruginosa and Bradyrhizobium sp., in the control of root infecting fungi and root knot nematode on urd bean Vigna mungo (L) Hepper" 12 : 35-41, 2000

29 Glick, B. R., "Biochemical and genetic mechanisms used by plant growth-promoting bacteria" Imperial College Press 134-179, 1999

30 Ryu, C. M., "Bacterial volatiles induce systemic resistance in arabidopsis" 134 : 1017-1026, 2004

31 Grichko, V.P., "Amelioration of flooding stress by ACC deaminase-containing plant growth-promoting bacteria" 39 : 11-17, 2001