계배 피부의 분화에 따른 형태학적 변화와 단백질 유형에 관한 연구 = Morphological Changes and Protein Patterns during Differentiation of the Chick Embryo Skin

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the morphological changes and protein pattern of the chick embryo skin during development. The results were as follows; Chick embryo skin of the 4th day after incubation was already discriminated between two layers: epidermis and dermis. The epidermis was consisted of 2 cell layers which were outer flattened and inner cuboidal. Intercelluar junctions, especially desmosomes, were prominent between the lateral plasma membranes of the neighboring cells and there were large intercelluar spaces. The epidermal cells showed the numerous free ribosomes and the well differentiated mitochondria in the cytoplasm.
In the case of the 7th day after incubation, chick embryo skin was more differentiated. The epidermis was composed of four cell layers, and it showed the intercellular spaces reduced And it appeared connective tissue cells and capillaries within the dermis. There were prominent keratohyaline granules and increased tonofibrils in the epidermal cell cytoplasm.
Immediately after hatching, infoldings of epidermal surface and increasing of cells in number were obvious. The cells in the stratum germinativum actively undertook mitosis. There were abundant tonofibrils in outer epidermis.
The protein banding pattern of chick embryo skin by the electrophoresis; the number of bands gradually increased until hatching, but they decreased after hatching.
In conclusion, differentiation of cells in the chick embryo skin was characterized by the increase of free ribosomes, the appearance of electron dense mitochondria, the active mitosis, the formation of intercelluar desmosome, the alteration of cell shapes, and the elevation of protein synthesis activity.