Effects of Scytosiphon lomentaria on osteoblastic proliferation and differentiation of MC3T3-E1 cells

다국어 초록 (Multilingual Abstract)

BACKGROUND/OBJECTIVES: Bone formation and bone resorption continuously occur in bone tissue to prevent the accumulation of old bone, this being called bone remodeling. Osteoblasts especially play a crucial role in bone formation through the differentiation and proliferation. Therefore, in this study, we investigated the effects of Scytosiphon lomentaria extract (SLE) on osteoblastic proliferation and differentiation in MC3T3-E1 cells.
MATERIALS/METHODS: A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining and protein expression analysis of osteoblastic genes were carried out to assess the osteoblastic proliferation and differentiation.
RESULTS: The results indicated that treatment of SLE promoted the proliferation of MC3T3-E1 cells and improved ALP activity. And, SLE treatment significantly promoted mineralized nodule formation compared with control. In addition, cells treated with SLE significantly upregulated protein expression of ALP, type 1 collagen, bone morphogenetic protein 2, runt-related transcription factor 2, osterix, and osteoprotegerin.
CONCLUSIONS: The results demonstrate that SLE promote differentiation inducement and proliferation of osteoblasts and, therefore may help to elucidate the transcriptional mechanism of bone formation and possibly lead to the development of bone-forming drugs.

목차 (Table of Contents)

• INTRODUCTION
• MATERIALS AND METHODS
• RESULTS
• DISCUSSION
• REFERENCES

참고문헌 (Reference)

1 Zhang R, "Wnt/β-catenin signaling activates bone morphogenetic protein 2 expression in osteoblasts" 52 : 145-156, 2013

2 Rodan GA, "Therapeutic approaches to bone diseases" 289 : 1508-1514, 2000

3 Ducy P, "The osteoblast: a sophisticated fibroblast under central surveillance" 289 : 1501-1504, 2000

4 O'Gorman DM, "The marinederived, multi-mineral formula, Aquamin, enhances mineralisation of osteoblast cells in vitro" 26 : 375-380, 2012

5 Niu YB, "The beneficial effect of Radix Dipsaci total saponins on bone metabolism in vitro and in vivo and the possible mechanisms of action" 23 : 2649-2660, 2012

6 Lee HS, "Stimulation of osteoblastic differentiation and mineralization in MC3T3-E1 cells by yeast hydrolysate" 25 : 716-723, 2011

7 Riggs BL, "Sex steroids and the construction and conservation of the adult skeleton" 23 : 279-302, 2002

8 Bai XC, "Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-kappaB" 314 : 197-207, 2004

9 Huang W, "Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells" 274 : 226-234, 2002

10 Simonet WS, "Osteoprotegerin: a novel secreted protein involved in the regulation of bone density" 89 : 309-319, 1997

11 Musiał K, "Osteopontin (OPN), PDGF-BB (platelet-derived growth factor) and BMP-7 (bone morphogenetic protein) as markers of atherogenesis in children with chronic kidney disease (CKD) treated conservatively--preliminary results" 24 (24): 25-27, 2008

12 Neve A, "Osteoblast physiology in normal and pathological conditions" 343 : 289-302, 2011

13 Hill PA, "Multiple extracellular signals promote osteoblast survival and apoptosis" 138 : 3849-3858, 1997

14 Park KH, "Melatonin promotes osteoblastic differentiation through the BMP/ERK/Wnt signaling pathways" 51 : 187-194, 2011

15 Bellows CG, "Initiation and progression of mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate" 14 : 27-40, 1991

16 Chandini SK, "In vitro antioxidant activities of three selected brown seaweeds of India" 107 : 707-713, 2008

17 Hsieh TP, "Icariin isolated from Epimedium pubescens regulates osteoblasts anabolism through BMP-2, SMAD4, and Cbfa1 expression" 17 : 414-423, 2010

18 Whyte MP., "Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization" 15 : 439-461, 1994

19 Nakamura T, "High tocopherol content in a brown alga Ishige okamurae" 60 : 793-794, 1994

20 Yonezawa T, "Harmine, a β-carboline alkaloid, inhibits osteoclast differentiation and bone resorption in vitro and in vivo" 650 : 511-518, 2011

21 W W., "Glial line-derived neurotrophic factor (GDNF): biological activities" 58 : 155-159, 1999

22 Michaëlsson K, "Genetic liability to fractures in the elderly" 165 : 1825-1830, 2005

23 Slemenda CW, "Genetic determinants of bone mass in adult women: a reevaluation of the twin model and the potential importance of gene interaction on heritability estimates" 6 : 561-567, 1991

24 Z.P. Liu, "Effects of trans-Resveratrol from Polygonum cuspidatum on Bone Loss Using the Ovariectomized Rat Model" 한국식품영양과학회 8 (8): 14-19, 2005

25 Horiguchi Y, "Effects of Bordetella bronchiseptica dermonecrotic toxin on the structure and function of osteoblastic clone MC3T3-E1 cells" 59 : 1112-1116, 1991

26 Li F, "Echinacoside promotes bone regeneration by increasing OPG/RANKL ratio in MC3T3-E1 cells" 83 : 1443-1450, 2012

27 Rassi CM, "Down-regulation of osteoclast differentiation by daidzein via caspase 3" 17 : 630-638, 2002

28 Yoon HJ, "Dichloromethane extracts of Sophora japonica L. stimulate osteoblast differentiation in mesenchymal stem cells" 33 : 1053-1062, 2013

29 Christiansen C., "Consensus development conference: diagnosis, prophylaxis, and treatment of osteoporosis" 94 : 646-650, 1993

30 Isomura H, "Bone metabolism and oxidative stress in postmenopausal rats with iron overload" 197 : 93-100, 2004

31 Phimphilai M, "BMP signaling is required for RUNX2-dependent induction of the osteoblast phenotype" 21 : 637-646, 2006

32 Demirel Z, "Antimicrobial and antioxidant activity of brown algae from the Aegean sea" 74 : 619-628, 2009

33 Kühn MC, "Adipocyte-secreted factors increase osteoblast proliferation and the OPG/RANKL ratio to influence osteoclast formation" 349 : 180-188, 2012

34 Lane NE, "A review of anabolic therapies for osteoporosis" 5 : 214-222, 2003

35 Tietz NW, "A reference method for measurement of alkaline phosphatase activity in human serum" 29 : 751-761, 1983