Cloning Vectors for Rice

다국어 초록 (Multilingual Abstract)

We developed various binary vectors that can be

used for expressing a foreign gene in rice. Vectors

pGA3426, pGA3436, and pGA3626 are intended for

overexpression of a gene using the maize Ubiquitin

promoter, whereas pGA3780 is for rather mild expression

of a gene using the rice Actin1 promoter. Vector pGA3777

is for expressing two genes simultaneously. We also

developed binary vectors for expressing a fusion protein

with a tag. Four vectors (pGA3427, pGA3428, pGA3429,

and pGA3438) are for protein tags with sGFP, HA, His, and

Myc, respectively. Vector pGA3383 is for analyzing

promoter activity using the GUS reporter. In this vector,

multiple cloning sites in front of GUS can be utilized for

accepting a promoter fragment. We also generated transient

expression vectors for studying the subcellular localization

of a protein. Vectors pGA3452, pGA3651, and pGA3652

are for GFP fusion; pGA3574 for RFP fusion; pGA3697 for

Myc tag; and pGA3698 for HA tag. In addition, we

generated pGA3506, pGA3516, pGA3592, and pGA3593

for facilitating the subcloning of full-length cDNA clones

into our binary vectors.

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