동시당화발효공정을 위한 바이오캡슐 형성 = Bio-capsule Formation for Synchronous Saccharification and Fermentation Process

국문 초록 (Abstract)

본 연구에서는 동시당화발효공정으로 바이오에탄올을 생산하기 위하여 바이오캡슐 형성을 시도하였다. 다수의 당화곰팡이 균주들과 발효 효모 균주들이 먼저 탐색되었다. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201및 P. chrysogenum KACC 44363이 α-amylase와 glucoamylase와 같은 당화 효소를 우수하게 생산하는 균주이었으며, Saccharomyces cerevisiae IFO-M-07이 조사된 균주 중에서 가장 에탄올 생산능이 높았다. 다음으로 pellet 형성 및 바이오 캡슐 형성을 위한 최적 조건을 평가하였다. 모든 조사된 곰팡이 모두 pellet을 형성하였으며, 바이오캡슐의 최적조건은 28℃, 120 rpm이었다. 최종적으로 형성된 바이오캡슐을 이용하여 동시당화발효를 수행하여, Aspergillus sp. BCNU 6200의 바이오캡슐(Aspergillus sp. BCNU 6200 +S. cerevisiae IFO-M-07)이 10일간 발효시 30℃, 120 rpm에서3.9%의 에탄올을 생산함을 확인하였다. 본 실험 결과는 동시 당화발효 공정으로 바이오에탄올을 생산하는데 있어서 바이오캡슐을 활용함에 관한 유용한 정보를 제공하고 있다.

다국어 초록 (Multilingual Abstract)

For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, bio-capsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as α-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were 28℃ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at 30℃ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.

참고문헌 (Reference)

1 하성민, "흑마늘 와인의 발효 및 그 특성" 한국생명과학회 26 (26): 796-804, 2016

2 김성준, "음식물 쓰레기 동시당화 발효에 의한 에탄올 생산" 한국생물공학회 21 (21): 474-478, 2006

3 강민경, "맥주 폐 효모액의 당화 및 에탄올 발효능" 한국화학공학회 51 (51): 709-715, 2013

4 김민식, "누룩곰팡이 분리균의 다양성 및 당화능 분석과 독소생산능 조사" 한국균학회 42 (42): 191-200, 2014

5 Hatami M, "Simultaneous saccharification and fermentation (SSF) of rice cooker wastewater by using Aspergillus niger and Saccharomyces cerevisiae for ethanol production" 2 : 103-107, 2015

6 김민지, "Saccharina japonica를 이용한 전처리 및 분리당화발효와 동시당화발효 로부터 에탄올 생산" 한국생물공학회 27 (27): 86-90, 2012

7 Balkan B, "Production and properties of α-amylase from Penicillium chrysogenum and its application in starch hydrolysis" 35 : 169-178, 2005

8 Lakshmi MC, "Production and optimization of glucoamylase from Aspergillus oryzae NCIM 1212 using wheat bran, varying chemical parameters under solid state fermentation" 3 : 70-76, 2014

9 Ha JH, "Potential of the waste from beer fermentation broth for bio-ethanol production without any additional enzyme, microbial cells and carbohydrates" 49 : 298-304, 2011

10 Nyman J, "Pellet formation of zygomycetes and immobilization of yeast" 30 : 516-522, 2013

11 Doraiswami Ramkrishna, "Issues with increasing bioethanol productivity: A model directed study" 한국화학공학회 27 (27): 576-586, 2010

12 Kourkoutas Y, "Immobilization technologies and support materials suitable in alcohol beverages production: a review" 21 : 377-397, 2004

13 Groboillot A, "Immobilization of cells for application in the food industry" 14 : 75-107, 1994

14 하정환, "Evaluation of sediments of the waste from beer fermentation broth for bioethanol production" 한국화학공학회 29 (29): 1224-1231, 2012

15 Fujii N, "Ethanol production from starch by immobilized Aspergillus awamori and Saccharomyces pastorianus using cellulose carriers" 27 : 52-57, 2001

16 Kadam KL, "Environmental benefits on a life cycle basis of using bagasse-derived ethanol as a gasoline oxygenate in India" 30 : 371-384, 2002

17 Kang SC, "Effects of loess on the mycelial pellet formation of phosphate dissolving fungus, Penicillium sp. GL-101 in the submerged culture" 14 : 337-341, 1999

18 Park SY, "Direct ethanol production from starch substrate by polyploid recombinant yeast secreting both alpha-amylase and glucoamylase" 24 : 604-612, 1996

19 García-Martínez T, "Co-culture of Penicillium chrysogenum and Saccharomyces cerevisiae leading to the immobilization of yeast" 86 : 812-817, 2011

20 Lee WS, "Bioethanol production from sweet potato by co-immobilization of saccharolytic molds and Saccharomyces cerevisiae" 39 : 216-222, 2012