소나무 배발생조직 라인 별 체세포배 유도 및 식물체 재분화 = Somatic Embryogenesis and Plant Regeneration with Embryogenic Tissue Lines in Pinus densiflora

국문 초록 (Abstract)

본 연구는 소나무의 배발생조직 라인 간 증식율, 체세포배 유도 및 발아율의 효과를 조사하기 위해 수행되었다. 소나무 미숙접합배로부터 유도된 15개 배발생조직 라인 간 증식율 비교에서 05-4 라인은 5.3배의 최대 증식율을 보인 반면, 05-37라인은 1.4배로 가장 저조하여 조직 라인 간 증식율 차이가 크게 나타났다. 배발생조직 13개 라인의 체세포배 유도에서는 05-4라인에서 39.8개(90 mg 조직기준)를 유도하여 가장 높았지만 전체적으로는 단지 5개 조직라인(05-4, 12, 21, 29 및 37)에서만 체세포배 유도가 가능하였다. 나머지 8개 라인에서는 전혀 유도되지 않아 조 직라인 간 체세포배 유도효율의 큰 차이를 보였다. 3개의 조직라인에서 유래한 체세포배의 발아비교에서 05-4라인 유래 체세포배 발아율이 0.2% gelrite에서 가장 높은 47.3%로 나타났으나, 05-4 및 29라인에서는 0.2% 이상의 gelrite 농도에서 발아율이 점차 감소하는 경향을 보였다. 한편 05-37 라인 유래 체세포배의 발아는 0.1 혹은 0.2% 농도에서는 전혀 발아가 이루어지지 않아 gelrite 농도에 따른 발아율 차이를 나타냈다. 결론적으로 소나무의 배발생조직 라인 간 증식율, 체세포배 발생 효율 및 발아효과는 배발생조직 라인에 따라 크게 좌우되는 경향을 보였다.

다국어 초록 (Multilingual Abstract)

This experiment was conducted to evaluate growth rate among 15 embryogenic tissue lines (ETLs), comparison of maturation on somatic embryos (SEs) with 13 ETLs and efficiency with various concentrations of gelrite on SEs germination in Japanese red pine (Pinus densiflora). In comparison of ETLs growth rate (folds) with 15 lines, the 05-4 line (5.3 folds) showed the highest rate, on the other hand, the lowest one was recorded in the line of 05-37 (1.4 folds). The 13 ETLs were tested for the extent of SEs production. The best production was recorded in the line of 05-4 (39.8/90 mg F.W.). However, most of ETLs, except 5 lines (05-4,12, 21, 29 and 37), did not produce SEs at all, therefore, big differences in the ability of SEs production existed among the ETLs tested. Effects of various gelrite concentrations for SEs germination with 3 ETLs were also compared. The highest result was obtained from 0.2% gelrite concentration with 05-4 line (47.3%), there was a inclination that the rate of germination was gradually declined over 0.2% gelrite concentration with the 05-4 and 29 lines. respectively. In contrast, in the line of 05-37, no SEs germination occurred on medium with 0.1 or 0.2% gelrite. In conclusion, the growth rate, SEs production and germination frequency were appeared to deeply depended on the ETLs.

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