To study the function of heterodimeric GABAB receptor, it is needed to construct the dicistronic vector for the simultaneous expression of GABAB1(GB1) and GABAB2(GB2) subtype genes. To do this, the internal ribosome entry sequence was used and succeed...
To study the function of heterodimeric GABAB receptor, it is needed to construct the dicistronic vector for the simultaneous expression of GABAB1(GB1) and GABAB2(GB2) subtype genes. To do this, the internal ribosome entry sequence was used and succeed the construction of two types of dicistronic vector of GABAB receptor with the living fluorescent color tag, pGA21 and pGA23. Its expression was confirmed in COS-1 cells. This vectors will be useful to elucidate the physiological role of GABAB receptors in central nervous system.