Paenibacillus filicis sp. nov., Isolated from the Rhizosphere of the Fern

다국어 초록 (Multilingual Abstract)

A Gram-positive and endospore-forming bacterial strain, designated S4T, was isolated from the rhizosphere
of ferns in Daejeon, Republic of Korea. This isolate is strictly aerobic, motile, and rod-like in shape, and
it is positive for catalase, oxidase, esterase lipase, and β-galactosidase activities. In addition, this strain
grows when cultured at temperatures between 15 and 37°C and at pH values ranging from 5.5 to 9.0.
The DNA G+C content was determined to be 53.2 mol%. Strain S4T has meso-diaminopimelic acid in the
cell-wall peptidoglycan; it also contains menaquinone 7 (MK-7) as the predominant isoprenoid quinone and
anteiso-C15:0 (57.5%), iso-C16:0 (11.3%), and C16:0 (9.4%) as the major cellular fatty acids. Phylogenetic
analysis based on alignments of the 16S rRNA gene sequence showed that S4T is affiliated with a cluster of
strains within the genus Paenibacillaceae and is most closely related to Paenibacillus chinjuensis WN9T,
with 96.8% similarity. Based on the phylogenetic and phenotypic characteristics of strain S4T, we believe
that this isolate should be distinguished from all type species of the genus Paenibacillus and should thus
represent a novel taxon within the genus Paenibacillus. We propose naming this type species Paenibacillus
filicis sp. nov. for the rhizosphere isolate; the type strain will be known as S4T (=KCTC 13693T =KACC
14197T =JCM 16417T).

참고문헌 (Reference)

1 Rivas, R., "Xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (Ulmus nigra)" 53 : 99-103, 2003

2 Shida, O, "Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus" 47 : 289-298, 1997

3 Chakrabarty, A.N, "The hydrolysis of Tween 80 by vibrios and aeromonads" 33 : 397-402, 1970

4 Cole, J.R, "The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy" 31 : 442-443, 2003

5 Thompson, J.D, "The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools" 25 : 4876-4882, 1997

6 Stackebrandt, E, "Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology" 44 : 846-849, 1994

7 Park, M.J, "Paenibacillus soli sp. nov., a xylanolytic bacterium isolated from soil" 57 : 146-150, 2007

8 Kim, D.S, "Paenibacillus elgii sp. nov., with broad antimicrobial activity" 54 : 2031-2035, 2004

9 Yoon, J.H, "Paenibacillus chinjuensis sp. nov., a novel exopolysaccharideproducing bacterium" 52 : 415-421, 2002

10 Heyndrickx, M, "Paenibacillus (formerly Bacillus) gordonae (Pichinoty et al. 1986) Ash et al. 1994 is a later subjective synonym of Paenibacillus (formerly Bacillus) validus (Nakamura 1984) Ash et al. 1994: emended description of P. validus" 45 : 661-669, 1995

11 Felsenstein,J, "PHYLIP (phylogeny inference package), version 3.5c"

12 Ash, C., "Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test" 64 : 253-260, 1993

13 Sambrook, J, "Molecular Cloning: a Laboratory Manual"

14 Euzeby,J.P, "List of bacterial names with standing in nomenclature: a folder available on the Internet" 47 : 590-592, 1997

15 Komagata, K, "Lipids and cell-wall analysis in bacterial systematics" 19 : 161-203, 1987

16 Shin, Y.K, "Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036 T" 6 : 68-69, 1996

17 Wayne, L.G, "International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics" 37 : 463-464, 1987

18 Sasser,M, "Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids" Inc., Newark 1990

19 Atlas,R.M, "Handbook of Microbiological Media" CRC Press 1993

20 Chun, J, "EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences" 57 : 2259-2261, 2007

21 Collins, M.D, "Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications" 45 : 316-354, 1981

22 Tamaoka, J, "Determination of DNA base composition by reversed-phase high-performance liquid chromatography" 25 : 125-128, 1984

23 Felsenstein,J, "Confidence limits on phylogenies: an approach using the bootstrap" 39 : 783-791, 1985

24 Kimura,M, "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences" 16 : 11-120, 1980

25 Weisburg, W.G., "16S ribosomal DNA amplification for phylogenetic study" 173 : 697-703, 1991