Molecular Typing in Public Health Laboratories: From an Academic Indulgence to an Infection Control Imperative

다국어 초록 (Multilingual Abstract)

Using three Austrian case studies, the variegated applications of molecular typing in today’s public health laboratories are discussed to help illustrate preventive management strategies relying on DNA subtyping. DNA macrorestriction analysis by pulsed field gel electrophoresis has become the gold standard for subtyping of food borne pathogens like listeria, salmonella, campylobacter and Bacillus cereus. Using a Salmonella Mbandaka outbreak from the year 2010 as example, it is shown how the comparison of patterns from human isolates, food isolates, animal isolates and feed isolates can allow to identify and confirm a source of disease. An epidemiological connection between the simultaneous occurrence of tuberculosis in cattle and deer with cases of human tuberculosis due to Mycobacterium caprae in 2010 was excluded using mycobacterial interspersed repetitive units variable-number tandem repeats subtyping. Also in 2010, multilocus sequence typing with nonselective housekeeping genes, the so-called sequence based typing protocol, was used to elucidate connections between an environmental source (a hospital drinking water system) and a case of legionellosis. During the last decades, molecular typing has evolved to become a routine tool in the daily work of public health laboratories. The challenge is now no longer to simply type microorganisms, but to type them in a way that allows for data exchange between public health laboratories all over the world.

참고문헌 (Reference)

1 Amt der Tiroler Landesregierung, "Tbc im Oberen Lechtal gemeinsam bekampfen"

2 Graves LM, "Subtying Listeria monocytogenes, In Listeria, listeriosis and food safety. 3rd ed" CRC Press 283-304, 2007

3 Van Embden JD, "Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology" 31 (31): 406-409, 1993

4 Ribot EM, "Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet" 3 (3): 59-67, 2006

5 Pavlic M, "Simultaneous infection with two drug-susceptible Mycobacterium tuberculosis strains in an immunocompetent host" 37 (37): 4156-4157, 1999

6 Schwartz DC, "Separation of yeast chromosomesized DNAs by pulsed-field gradient gel electrophoresis" 37 (37): 67-75, 1984

7 Cohen ML, "Resurgent and emergent disease in a changing world" 54 (54): 523-532, 1998

8 Amt der Vorarlberger Landesregierung, "Resume zum Jagdjahr 2010/2011"

9 Croucher NJ, "Rapid pneumococcal evolution in response to clinical interventions" 331 (331): 430-434, 2011

10 Gerner-Smidt P, "PulseNet USA: a five-year update" 3 (3): 9-19, 2006

11 Vincent V, "Mycobacterium: phenotypic and genotypic identification, In Manual of clinical microbiology. 8th ed" ASM Press 560-584, 2003

12 Lindstedt BA, "Multiple-locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria" 26 (26): 2567-2582, 2005

13 Enright MC, "Multilocus sequence typing" 7 (7): 482-487, 1999

14 Sougakoff W, "Molecular epidemiology of multidrugresistant strains of Mycobacterium tuberculosis" 17 (17): 800-805, 2011

15 Rotwild im AuBerfern von TBC befallen, "MaBnahmen notwendig"

16 Hoorfar J, "Future trends in rapid methods: where is the field moving, and what should we focus on?, In Rapid detection, characterization, and enumeration of foodborne pathogens" ASM press 413-420, 2011

17 He M, "Evolutionary dynamics of Clostridium difficile over short and long time scales" 107 (107): 7527-7532, 2010

18 Hunter SB, "Establishment of a universal size standard strain for use with the PulseNet standardized pulsed-field gel electrophoresis protocols: converting the national databases to the new size standard" 43 (43): 1045-1050, 2005

19 Fry NK, "Designation of the European Working Group on Legionella Infection (EWGLI) amplified fragment length polymorphism types of Legionella pneumophila serogroup 1 and results of intercentre proficiency testing using a standard protocol" 21 (21): 722-728, 2002

20 Gaia V, "Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila" 43 (43): 2047-2052, 2005

21 Prodinger WM, "Characterization of Mycobacterium caprae isolates from Europe by mycobacterial interspersed repetitive unit genotyping" 43 (43): 4984-4992, 2005

22 Salgado-Voss AS, "Bericht uber Zoonosen und ihre Erreger in osterreich im Jahr 2010"

23 Nindler P, "Bauern fordern Tbc-Freiheit: Rotwild vor Totalabschuss"

24 Berger S, "Bakterien in Krieg und Frieden: eine Geschichte der medizinischen Bakteriologie in Deutschland 1890-1933" Wallstein Verlag 143-170, 2009

25 European Centre for Disease Prevention and Control, "Annual report of the director 2010" European Centre for Disease Prevention and Control 13-, 2010

26 Ratzow S, "Addition of neuA, the gene encoding N-acylneuraminate cytidylyl transferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains" 45 (45): 1965-1968, 2007

27 최금발, "2010년 일개 회사에서 집단발생한 바실루스 세레우스 식중독에 관한 역학조사" 대한예방의학회 44 (44): 65-73, 2011