食鹽水 또는 蒸溜水 投與가 副腎皮質에 미치는 影響에 關한 電子顯微鏡的 硏究 = Ultrastructural Changes of Adrenal Cotex of Rats Substituting 2% Sodium Chloride and Distilled Water in Lieu of Drinking Water

다국어 초록 (Multilingual Abstract)

It is well known that various degrees and combinations of regressive changes and hemorrhagic disorders of different organs may be observed in sodium chloride intoxication. Reports on the ultrastructural changes of adrenal cortex in sodium chloride intoxication are rare, however.
An attempt was made to study alterations of fine structures of the adrenal cortex by light and electron microscopy.
Twenty albino rates, maintained on a stock diet, weighing from 160 to 310 gm, were divided into 3 groups.
Group 1. Control group.
2 rats: tap water was given as drinking water. Total daily NaCl intake, 2.2mEq.
Group 2.
9 rats: a 2% NaCl solution was substituted for drinking water. Total daily NaCl intake, 25.0 mEq.
Group 3.
9 rats: distilled water was substituted for drinking water. Total daily NaCl intake, 1.0mEq.
Three rats were sacrificed at the end of the lst, 2nd and 3rd experimental weeks from groups 2 and 3. The control group was sacrificed similarly. Adrenal glands were extirpated and studied by both light and electron microscopic examinations.
The results were as follows:
1. Considerable weight loss was observed in the group of rats given the 2% NaCl solution (group 2), whereas retarded weight increase was noted in the experimental group of rats given distilled water (group 3).
2. In the zona glomerulosa of the adrenal cortex, a marked atrophic change for the group of rats on the 2% NaCl solution (group 2) was noted and a mild hydropic swelling of the cytoplasms of the cells of the zona glomerulosa on the group of rats on the distilled water (group 3) was observed.
3. Electron microscopic study revealed that a degenerative change occurred on the group of rats on the 2% NaCl solution (group 2), characterized by and atrophy of sER and the Golgicomplex; and increased number of lipid droplets, lysosome and glycogen particles; also a swelling of the mitochondrial and a decreased number of mitochondrial cristae; plus a chondriosphere formation. On the other hand, the group of rats on the distilled water (group 3), had a proliferative change such as an increased number of sER, with the dilatation of Golgi complex and both a decreased number of lipid droplets and glycogen particles.
It can be concluded, therefore:
The functional activity of the intracellular organells of the zona glomerulosa of the adrenal glands responsible for synthesis and secretion of mineralocorticoid was depressed when the experimental rats were given 2% NaCl solution as a substitute for drinking water after a considerable length of time. This functional activity was accelerated when the rats were given distilled water in lieu of drinking water over long duration.