Proteomics for drug discovery : methods and protocols

목차 (Table of Contents)

• CONTENTS
• Preface = v
• Contributors = ix
• 1 A Photoaffinity Labeling-Based Chemoproteomics Strategy for Unbiased Target Deconvolution of Small Molecule Drug Candidates / Jason R. Thomas ; Scott M. Brittain ; Jennifer Lipps ; Luis Llamas ; Rishi K. Jain ; Markus Schirle = 1
• 2 Multiplexed Liquid Chromatography-Multiple Reaction Monitoring Mass Spectrometry Quantification of Cancer Signaling Proteins / Yi Chen ; Kate J. Fisher ; Mark Lloyd ; Elizabeth R. Wood ; Domenico Coppola ; Erin Siegel ; David Shibata ; Yian A. Chen ; John M. Koomen = 19
• 3 Monitoring Dynamic Changes of the Cell Surface Glycoproteome by Quantitative Proteomics / Mathias Kalxdorf ; Hans Christian Eberl ; Marcus Bantscheff = 47
• 4 High-Resolution Parallel Reaction Monitoring with Electron Transfer Dissociation for Middle-Down Proteomics: An Application to Study the Quantitative Changes Induced by Histone Modifying Enzyme Inhibitors and Activators / Michael J. Sweredoski ; Annie Moradian ; Sonja Hess = 61
• 5 Preparation and Immunoaffinity Depletion of Fresh Frozen Tissue Homogenates for Mass Spectrometry-Based Proteomics in the Context of Drug Target／Biomarker Discovery / DaRue A. Prieto ; King C. Chan ; Donald J. Johann Jr. ; Xiaoying Ye ; Gordon Whitely ; Josip Blonder = 71
• 6 Target Identification Using Cell Permeable and Cleavable Chloroalkane Derivatized Small Molecules / Jacqui L. Mendez-Johnson ; Danette L. Daniels ; Marjeta Urh ; Rachel Friedman Ohana = 91
• 7 Microfluidics-Mass Spectrometry of Protein-Carbohydrate Interactions : Applications to the Development of Therapeutics and Biomarker Discovery / Alina D. Zamfir = 109
• 8 Studying Protein-Protein Interactions by Biotin AP-Tagged Pulldown and LTQ-Orbitrap Mass Spectrometry / Zhongqiu Xie ; Yuemeng Jia ; Hui Li = 129
• 9 Post-Translational Modification Profiling-Functional Proteomics for the Analysis of Immune Regulation / Avital Eisenberg-Lerner ; Ifat Regev ; Yifat Merbl = 139
• 10 Reverse Phase Protein Arrays and Drug Discovery / Kenneth G. Macleod ; Bryan Serrels ; Neil O. Carragher = 153
• 11 Probing Protein Kinase-ATP Interactions Using a Fluorescent ATP Analog / Leslie E. W. LaConte ; Sarika Srivastava ; Konark Mukherjee = 171
• 12 Preparation of Disease-Related Protein Assemblies for Single Particle Electron Microscopy / A. Cameron Varano ; Naoe Harafuji ; William Dearnaley ; Lisa Guay-Woodford ; Deborah F. Kelly = 185
• 13 Identification of Lipid Binding Modulators Using the Protein-Lipid Overlay Assay / Tuo-Xian Tang ; Wen Xiong ; Carla V. Finkielstein ; Daniel G.S. Capelluto = 197
• 14 Resazurin Live Cell Assay : Setup and Fine-Tuning for Reliable Cytotoxicity Results / José Á. Rodríguez Corrales ; Jatinder S. Josan = 207
• 15 Exploring Protein-Protein Interactions as Drug Targets for Anti-cancer Therapy with In Silico Workflows / Alexander Goncearenco ; Minghui Li ; Franco L. Simonetti ; Benjamin A. Shoemaker ; Anna R. Panchenko = 221
• 16 Method to Identify Silent Codon Mutations That May Alter Peptide Elongation Kinetics and Co-translational Protein Folding / Ronald Worthington ; Elijah Ball ; Brentsen Wolf ; Gregory Takacs = 237
• 17 In Silico Design of Anticancer Peptides / Shailesh Kumar ; Hui Li = 245
• 18 Docking and Virtual Screening in Drug Discovery / Maria Kontoyianni = 255
• 19 Bioinformatics Resources for Interpreting Proteomics Mass Spectrometry Data / Julia M. Lazar = 267
• Erratum to : Probimg Protein Kinase-ATP Interactions Using a Fluorescent ATP Analog = E1
• Index = 297