Temporal changes of nutrient composition from pollen patty to bee bread with special emphasis on amino and fatty acids composition

다국어 초록 (Multilingual Abstract)

Bee bread is prepared from pollen sources and salivary secretions by honey bee workers to serves the nutritional purpose of colony members. However, changes in nutrient composition occurring in bee bread from the pollen sources including pollen patty are poorly understood. We, therefore, examined the nutrient contents of pollen patty, used as source pollen feed of honey bee colony, and bee bread prepared by honey bee from this source at different time interval. Results revealed that amino acid content was increased from pollen patty to bee bread but fatty acid content was decreased. Essential amino acids like valine, lysine, and nonessential amino acids such as proline and alanine increased significantly over time. Among fatty acids, oleic acid, linoleic acid and linolenic acid decreased significantly from pollen patty to bee bread. Minerals contents in bee bread did not show any significant change during the study period. These changes could be presumably justified due to addition of nectar, honey, gland secretions and also microbial symbionts which remains as a task of further study.

참고문헌 (Reference)

1 Crailsheim, K., "The protein balance of the honey bee worker" 21 : 417-429, 1990

2 Vasquez, A., "The lactic acid bacteria involved in the production of bee pollen and bee bread" 48 : 189-195, 2009

3 Wyatt, G. R., "The biochemistry of insect haemolymph" 6 : 75-102, 1961

4 Standifer, L. N., "Supplemental feeding of honey bee colonies" United States Department of Agriculture 1977

5 Judd, T. M., "Social insects:structure, function and behaviour" Nova 1-24, 2011

6 Urcan, A. C., "Similarity of data from bee bread with the same taxa collected in India and Romania" 23 : 2491-, 2018

7 Roy, R., "Review : Nectar biology : From molecules to ecosystem" 262 : 148-164, 2017

8 Shinbo, H., "Purification and characterisation of a proline-rich secretary protein that is a precursor to a structural protein of an insect spermatophore" 262 : 4794-4799, 1987

9 de Groot, A. P., "Protein and amino acid requirements of the honeybee Apis mellifera" 3 : 197-285, 1953

10 Teulier, L., "Proline as a fuel for insect flight : enhancing carbohydrate oxidation in hymenopterans" 283 : 20160333-, 2016

11 Dharampal, P. S., "Pollen-borne microbes shape fitness" 286 : 20182894-, 2019

12 Tajabadi, N., "Optimization of γ-aminobutyric acid production by Lactobacillus plantarum Taj-Apis362 from honeybees" 20 : 6654-6669, 2015

13 Arien, Y., "Omega-3 deficiency impairs honey bee learning" 112 : 15761-15766, 2015

14 A. O. A. C., "Official methods of analysis, 15th edition"

15 Guedegbe, H. J., "Occurrence of fungi in combs of fungus-growing termites(Isoptera : Termitidae, Macroterminae)" 113 : 1039-1045, 2009

16 ghosh sampat ; 정철의, "Nutritional value of bee-collected pollens of hardy kiwi, Actinidia arguta (Actinidiaceae) and oak, Quercus sp. (Fagaceae)" 한국응용곤충학회 20 (20): 245-251, 2017

17 Rogala, R., "Nutritional value for bees of pollen substitute enriched with synthetic amino acids. Part II. Biological methods" 48 : 29-36, 2004

18 Ma, L., "Nutritional effect of alphalinolenic acid on honey bee colony development (Apis mellifera L.)" 59 : 63-72, 2015

19 Donkersley, P., "Nutritional composition of honey bee food stores vary with floral composition" 185 : 749-761, 2017

20 Avni, D., "Nutritional aspects of honey bee-collected pollen and constraints on colony development in the eastern Mediterranean" 69 : 65-73, 2014

21 Nicolson, S. W., "Nectaries and Nectar" Springer 215-264, 2007

22 Tajabadi, N., "Molecular identification of Lactobacillus spp. isolated from the honey comb of the honey bee (Apis dorsata) by 16s rRNA gene sequencing" 52 : 235-241, 2013

23 Herbert Jr., E.W., "Mineral requirements for brood rearing by honeybees fed a synthetic diet" 17 : 118-122, 1978

24 Somerville, D. C., "Mineral content of honeybee-collected pollen from southern New South Wales" 42 : 1131-1136, 2002

25 Kostic, A. Z., "Mineral content of bee pollen from Serbia" 66 : 251-258, 2015

26 Gilliam, M., "Microbiology of pollen and bee bread : taxonomy and enzymology of molds" 20 : 53-68, 1989

27 Ghosh, S., "Metagenomics and Microbial Ecology Techniques and Applications" CRC Press 105-116, 2021

28 "Korean Food Standard Codex"

29 Barene, I., "Investigation of beebread and development of its dosage forms" 21 : 16-22, 2015

30 Dadd, R. H., "Insect nutrition : current developments and metabolic implications" 18 : 381-420, 1973

31 Arrese, E. L., "Insect fat body : energy, metabolism, and regulation" 55 : 207-225, 2010

32 Nation, J. L., "Insect Physiology and Biochemistry" CRC Press 2008

33 Omar, E., "Influence of different pollen diets on the development of hypopharyngeal glands and size of acid gland sacs in caged honey bees(Apis mellifera)" 48 : 425-436, 2017

34 Micheu, S., "Importance of proline and other amino acids during honeybee flight" 18 (18): 157-175, 2000

35 Klein, S., "Honey bees increase their foraging performance and frequency of pollen trips through experience" 9 : 6778-, 2019

36 Anderson, K. E., "Hive-stored pollen of honey bees : many lines of evidence are consistent with pollen preservation, not nutrient conversion" 23 : 5904-5917, 2014

37 Sampat Ghosh ; 전혜진 ; 정철의, "Foraging behaviour and preference of pollen sources by honey bee (Apis mellifera) relative to protein contents" 한국생태학회 44 (44): 26-32, 2020

38 Borghi, M., "Floral Metabolism of Sugars and Amino Acids : Implications for Pollinators’ Preferences and Seed and Fruit Set" 175 (175): 1510-1524, 2017

39 Stanley-Samuelson, D. W., "Fatty acids in insects : composition, metabolism, and biological significance" 9 : 1-33, 1988

40 Ceksteryte, V., "Fatty acid profiles of monofloral clover beebread and pollen and proteomics of red clover(Trifolium pratense)pollen" 80 : 2100-2108, 2016

41 Kaplan, M., "Fatty acid and proximate composition of bee bread" 54 : 497-504, 2016

42 Smith, M. R., "Effects of decreases of animal pollinators on human nutrition and global health : a modelling analysis" 386 : 1964-1972, 2015

43 Darvishzadeh, A., "Effect of proline as a nutrient on hypopharyngeal glands during development of Apis mellifera(Hymenopera : Apidae)" 4 : 137-143, 2015

44 Disayathanoowat, T., "Different dynamics of bacterial and fungal communities in hive-stored bee bread and their possible roles : A case study from two commercial honey bees in China" 8 : 264-, 2020

45 Negri, P., "Dietary supplementation of honey bee larvae with arginine and abscisic acid enhances nitric oxide and granulocyte immune responses after trauma" 8 : 85-, 2017

46 Kelly, M. A., "Diet fatty acid profile, membrane composition and lifespan : An experimental study using the blowfly(Calliphora stygia)" 138 : 15-25, 2014

47 Tajabadi, N., "Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata" 42 : 642-649, 2011

48 Sampat Ghosh ; 정철의, "Contribution of insect pollination to nutritional security of minerals and vitamins in Korea" 한국응용곤충학회 21 (21): 598-602, 2018

49 Beenakkers, A. M. T., "Comprehensive Insect Physiology, Biochemistry and Pharmacology, vol. 10" Pergamon Press 451-486, 1985

50 García, M. T. A., "Composition of the floral nectar of different subgenera of Argentinian Passiflora species" 283 : 133-147, 2009

51 Ceksteryte, V., "Composition and content of fatty acids in bee bread of various floral origin, collected in Lithuania and prepared for storage in different ways" 60 : 57-61, 2012

52 Abe, T., "Comparative study of the composition of hornet larval saliva, its effect on behaviour and role of trophallaxis" 99 : 79-84, 1991

53 vanEngelsdorp, D., "Colony collapse disorder : A descriptive study" 4 : e6481-, 2009

54 Urcan, A., "Chemical composition and biological activities of beebreadreview" 74 : 2017

55 Ghosh Sampat ; Jung Chuleui, "Changes in nutritional composition from bee pollen to pollen patty used in bumblebee rearing" 한국응용곤충학회 23 (23): 701-708, 2020

56 Haeder, S., "Candicidin-producing Streptomyces support leaf-cutting ants to protect their fungus garden against the pathogenic fungus Escovopsis" 106 : 4742-4746, 2009

57 Anderson, S. O., "Biochemistry of insect cuticle" 24 : 29-59, 1979

58 Ivaniˇsov´a, E., "Bee bread-perspective source of bioactive compounds for future" 9 : 592-598, 2015

59 Basualdo, M., "Bee bread increases honeybee haemolymph protein and promote better survival despite of causing higher Nosema ceranae abundance in honeybees" 6 : 396-400, 2014

60 Kiya, T., "Analysis of GABAergic and non-GABAergic neuron activity in the optic lobes of the forager and reorienting worker honeybee (Apis mellifera L.)" 5 : e8833-, 2010

61 Crailsheim, K., "Amino acids in honeybee worker haemolymph" 13 : 141-153, 1997

62 Nepi, M., "Amino acids and protein profile in floral nectar : Much more than a simple reward" 207 : 475-481, 2012

63 Bertazzini, M., "Amino acid content and nectar choice by forager honeybees (Apis mellifera L.)" 39 : 315-318, 2010