Development of Self-triggered MgO@MnOx Nanozyme for Enhanced ROS Scavenging = 활성산소 제거를 위한 자체-활성화 MgO@MnOx 나노자임 개발

목차 (Table of Contents)

• ABSTRACT ........................................................................................................................... i
• 국문 초록 ............................................................................................................................. iii
• TABLE OF CONTENTS ..................................................................................................... vi
• LIST OF FIGURES............................................................................................................ viii
• CHAPTER 1. INTRODUCTION ......................................................................................... 1
• 1.1 Reactive Oxygen Species (ROS)........................................................................... 1
• 1.1.1 The Role of ROS ...................................................................................... 1
• 1.1.2 ROS Scavenging Activity......................................................................... 1
• 1.2 Nanozymes for ROS Scavenging .......................................................................... 2
• 1.3 The Strategies of The Study .................................................................................. 2
• CHAPTER 2. MATERIALS AND METHODS................................................................... 5
• 2.1 Materials ................................................................................................................ 5
• 2.2 Synthesis of Nanomaterials ................................................................................... 5
• 2.3 Structural and Chemical Characterization............................................................. 6
• 2.4 Catalytic Activity and Electrochemical Measurements ........................................ 7
• 2.4.1 SOD-mimetic Activity Assay ................................................................... 7
• 2.4.2 CAT-mimetic Activity Assay ................................................................... 7
• 2.5 Energy Barrier Simulation..................................................................................... 7
• 2.6.1 Energy Barrier Simulation........................................................................ 8
• 2.6.2 Cyclic Voltammetry.................................................................................. 8
• 2.6 In Vitro Assays ...................................................................................................... 8
• 2.6.1 Cell Culture............................................................................................... 8
• 2.6.2 Cell Viability Assay.................................................................................. 9
• 2.6.3 Intracellular H2O2 Decomposition Assay ................................................. 9
• 2.6.4 Cell Migration Assay................................................................................ 9
• 2.6.5 Tube Formation Assay............................................................................ 10
• 2.7 In Vivo Studies .................................................................................................... 10
• 2.7.1 In Vivo Biocompatibility Evaluation...................................................... 10
• 2.7.2 Anti-Inflammatory Evaluation................................................................ 10
• 2.7.3 Immunohistochemistry ........................................................................... 11
• CHAPTER 3. RESULTS AND DISCUSSION .................................................................. 12
• 3.1 Synthesis and Characterization of MgO@MnOx Nanoparticles ......................... 12
• 3.2 Synergistic Effects of MgO and MnOx in ROS Scavenging ............................... 25
• 3.3 Mechanistic Insights into Enhanced Catalytic Activity ...................................... 29
• 3.3.1 Enhanced Catalytic Activity via Incorporation of Magnesium Oxide ... 29
• 3.3.2 Enhanced Catalytic Activity via Repositioning Magnesium Ions.......... 34
• 3.4 In Vitro Assessment of Cell Viability and ROS Scavenging Ability.................. 38
• 3.5 Investigation of Angiogenic Potential through ROS Scavenging in HUVECs... 43
• 3.6 Biocompatibility and Anti-inflammatory Evaluation.......................................... 49
• CHAPTER 4. CONCLUSION............................................................................................ 58
• REFERENCES.................................................................................................................... 59